TABLE 1 .

Functional metagenomic libraries constructed with DNA from dairy cow manure

Cow no.	Library name	Library type	No. of clones	Average insert size (kb)	Gb of cloned DNA	Antibiotic treatment history between September 2011 and March 2012
01	Cow01_fos	Fosmid	90,000	24.1	2.1	No treatment
	Cow01_si	Small insert	480,000	3.2	2.5
02	Cow02_fos	Fosmid	80,000	29.1	2.3	No treatment
	Cow02_si	Small insert	1,044,780	2.5	2.6
03	Cow03_fos	Fosmid	60,000	28.1	1.6	Repeatedly treated prior to sampling: January 2012, 125 mg ceftiofur, 5×; February 2012, 200 mg cephapirin, 20×
	Cow03_si	Small insert	1,427,800	3.1	4.3
04	Cow04_before_fos	Fosmid	60,000	32.3	1.9	No antibiotic treatment history; sample obtained before antibiotic treatment
	Cow04_before_si	Small insert	1,304,000	2.1	2.8
	Cow04_after_fos	Fosmid	142,800	31.3	4.4	Sample obtained after antibiotic treatment in March 2012: 125 mg ceftiofur, 5×
	Cow04_after_si	Small insert	1,428,000	2.3	1.4
Total	10 libraries		6,117,380		25.9