Skip to main content
. Author manuscript; available in PMC: 2015 Apr 9.
Published in final edited form as: Oncogene. 2013 Oct 21;33(41):4932–4940. doi: 10.1038/onc.2013.435

Figure 5.

Figure 5

NKG2D enhances intravasation and micrometastasis in the orthotopic SUM149PT xenotransplant model. (a) Two micrograph pairs of SUM149PT–TF serial tumor tissue sections stained for mouse endothelial CD31, or human pan-cytokeratin AE1/AE3 as human tumor cell marker. Micrograph pairs were derived from run 1 and run 2 experiments and document examples of human tumor cells located within cross sections of CD31-positive peritumoral vessels. (b) Schematic representation of presence of absence of GFP signals in lungs explanted from the run 1 and run 2 experimental and control hosts. Green boxes represent positive GFP signals revealed by Typhoon Trio scanning. (c) Four micrograph pairs of serial lung tissue sections from run 1 SUM149PT–TF tumor hosts stained for H&E or human pan-cytokeratin AE1/AE3. Each two micrograph pairs were derived from two different mice and show examples of micrometastasic clusters of AE1/AE3-positive cells. Numbers in bottom left micrograph corners indicate microscopic magnification; numbers in bottom right micrograph corners specify mouse identifications (mIDs).