Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Staphylococcus aureus | ||
| RN4220 | Mcs; restriction negative | Novick (1991) |
| COL | Homogenous Mcr (MIC, 1600 μg mL−1); Ems | Rockefeller University Collection |
| Escherichia coli | ||
| DH5α | recA endA1 gyrA96 thi-1 hsdR17 supE44 relA1 ϕ80 ΔlacZΔM15 | Invitrogen |
| BL21(DE3) | F–ompT gal dcm lon hsdSB(rB−mB−) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | Invitrogen |
| Plasmids | ||
| pET28a(+) | E. coli expression vector | Novagen |
| pET-AMR1–3GL | pET28a(+) expressing AMR1-3GL as a N-terminal His-tag fusion (fragment amplified with Pexp1 and Pexp4) | This study |
| pET-AMR1–2 | pET28a(+) expressing AMR1-2 protein as a N-terminal His-tag fusion. pET-AMR1-3GL was used to insert a stop codon by directed mutagenesis with primers Pexp_stop1 and Pexp_stop2 | This study |
| pET-AM | pET28a(+) expressing AM as a N-terminal His-tag fusion (fragment amplified with Pexp1 and Pexp5) | This study |
| pET-R3GL | pET28a(+) expressing R3GL as a N-terminal His-tag fusion (fragment amplified with Pexp2 and Pexp4) | This study |
| pET-GL | pET28a(+) expressing GL as a N-terminal His-tag fusion (fragment amplified with Pexp3 and Pexp4) | This study |
| pET-GLN | pET28a(+) expressing GLN as a N-terminal His-tag fusion (fragment amplified with Pexp3 and Pexp7) | This study |
| pET-GLC | pET28a(+) expressing GLC as a N-terminal His-tag fusion (fragment amplified with Pexp6 and Pexp4) | This study |