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Competitive binding assay for compound 21. FITC-labeled compound 5 was incubated with different concentrations of compound 21 with BT-474 cells. Cells were washed and fluorescence was detected using a plate reader. Ex λ 485 nm, Em λ 535 nm. A decrease in the fluorescence indicates binding of compound 21 to BT-474 cells expressing HER2 protein. As a control binding of FITC-5 is also shown.
