Figure 4.

Effect of anti-vimentin antibody on flow-dependent platelet adhesion to fibrin(ogen). Whole blood containing sheep IgG molecule or sheep anti-vim antibody was mixed with ristocetin (0.5 mg/mL) and perfused over surfaces coated with fibrin(ogen) at a shear rate of 1500 seconds⁻¹. After a 2-minute perfusion, the plates were washed with buffer, and attached platelets were recorded and quantified as described in “Methods.” Photomicrographs represent 3 separate experiments. The bar graph shows the percent of surface covered by firmly adhered platelets in 15 fields of view (mean ± SD, *P < .01).