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. Author manuscript; available in PMC: 2015 Apr 16.
Published in final edited form as: Oncogene. 2013 Oct 28;33(42):4985–4996. doi: 10.1038/onc.2013.444

Figure 6. Aurora-A induces ERα transactivation and upregulates CCND1 through phosphorylation of ERα-Ser167/Ser305.

Figure 6

(A) Luciferase assay. MDA-MB231 cells were transfected with indicated plasmids. Following 48 hours of incubation, luciferase assay was performed as described in Figure 4. (B and C) Semi-quantitative RT-PCR (upper panels) and Western blot (lower panels) analyses were carried out in Aurora-A transfected MCF7 and Aurora-A-knockdown BT474 cells. (D–F) ERα-negative MDA-MB-231 cells were transfected with indicated plasmids and subjected to RT-PCR and Western blot (D), luciferase (E) and ChIP (F) assays.