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. 1996 Feb 6;93(3):1119–1124. doi: 10.1073/pnas.93.3.1119

Protein-protein interactions in eukaryotic transcription initiation: structure of the preinitiation complex.

H Tang 1, X Sun 1, D Reinberg 1, R H Ebright 1
PMCID: PMC40041  PMID: 8577725

Abstract

We have used alanine scanning to analyze protein-protein interactions by human TATA-element binding protein (TBP) within the transcription preinitiation complex. The results indicate that TBP interacts with RNA polymerase II and general transcription factors IIA, IIB, and IIF within the functional transcription preinitiation complex and define the determinants of TBP for each of these interactions. The results permit construction of a model for the structure of the preinitiation complex.

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