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. 2014 Mar 24;53(14):2423–2432. doi: 10.1021/bi500158w

Figure 1.

Figure 1

Formation of metastable soluble non-native oligomers of metal-free SOD1. (A) Positions of the glutathione modification and of the FALS-linked amino acid substitutions included in the current study; residue positions are indicated by colored spheres on the background of the WT SOD1 crystal structure (PDB ID: 1spd). (B) SEC chromatograms showing aggregation of 100 μM apo-WT SOD1 at 37 °C in 20 mM Tris and 150 mM NaCl at pH 7.4 for up to 1 week. (C) Aggregation of 100 μM apo-SOD1 with the indicated FALS-linked amino acid substitutions under identical conditions as those described for B. Insets show chromatograms with expanded y-axes. (D) Weight average molar masses of metastable soluble SOD1 oligomers separated by SEC, as determined by multiangle light scattering (SEC-MALS). Black curves, absorbance at 280 nm (A280) vs elution volume (Ve); colored curves, molecular weight (MW) calculated at each Ve from the intensities of scattered light at multiple fixed detectors. Dashed gray lines indicate approximate theoretical molecular masses for SOD1 oligomers.