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CD spectra of Ac-TKAAAAKAAAAKAAGY-NH2 peptides with threonine modifications [unmodified Thr (free hydroxyl), ThrOGlcNAc, ThrOPO3H– (pH 4), and ThrOPO32– (pH 8)]: unmodified Thr (green squares), dianionic phosphothreonine (pH 8) (red circles), monoanionic phosphothreonine (pH 4) (open magenta circles), and ThrOGlcNAc (blue diamonds). CD experiments were conducted in water with 10 mM phosphate (pH 7 or as indicated) and 25 mM KF at 0.5 °C.
