Figure 2.

Specificity of CD8 T-cell clones. Each T-cell clone was activated with irradiated (2000 rads) immune splenocytes mock-pulsed, UV-inactivated Chlamydia muridarum (uvMoPn)-pulsed, and elementary bodies-depleted antigen (sAg)-pulsed. At 36 hr culture supernatants were harvested and [³H]thymidine was added; wells were harvested at 48 hr to score proliferation. (a) Proliferation in counts per minute (CPM). (b) Interferon-γ (IFN-γ) production as determined by ELISA. Data are means and SD for one experiment performed as quadruplicates. For each T-cell clone the experimental wells were compared with their mock-pulsed control and with the antigen-presenting-cells-only control (No T) for the relevant antigen. The higher P-value of those two comparisons (the least significant) was assigned and graphed. **P < 0·005; ***P < 0·0005.