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. 1989 Mar;8(3):805–813. doi: 10.1002/j.1460-2075.1989.tb03441.x

The product of a novel growth factor activated gene, fos B, interacts with JUN proteins enhancing their DNA binding activity.

M Zerial 1, L Toschi 1, R P Ryseck 1, M Schuermann 1, R Müller 1, R Bravo 1
PMCID: PMC400877  PMID: 2498083

Abstract

We have identified a gene, fos B, encoding a nuclear protein of 338 amino acids presenting a 70% homology with c-fos, whose expression is activated during G0/G1 transition. Growth factor stimulation of quiescent cells leads to a rapid and transient accumulation of fos B mRNA, with kinetics similar to those of c-fos. The induction of fos B mRNA levels is in part due to a dramatic increase in the transcription of the gene. The half-life of fos B mRNA is in the order of 10-15 min. Both transcriptional activation and mRNA stability are substantially increased in the presence of protein synthesis inhibitors. Immunoprecipitation studies showed that fos B as c-fos protein, forms a complex in vitro with c-jun and jun B proteins in the absence of a target binding sequence. Gel retardation assays demonstrated that fos B protein positively influences the binding of c-jun and jun B proteins to an AP-1 binding consensus sequence, suggesting that fos B protein plays a role in control of gene expression.

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