Table 2.
XG-19 and XG-20 HMCLs were cultured at 105 cells/ml without cytokine or with IL-6 (2 ng/ml), or with 104 osteoclasts with or without anti-IL-6 (BE8) MoAb (10 mg/ml), TACI-Fc (20 mg/ml), anti-IGF-1R MoAb (4 mg/ml), anti-IL-10 MoAb (10 mg/ml) or anti-CCR2 MoAb (10 mg/ml). Cells were recovered after 3 days of culture and apoptotic cells were detected by annexin V staining. Results are the means ± SD of five experiments.
| XG-20 | XG-19 | |||
|---|---|---|---|---|
| % Annexin V | P value (N = 5) | % Annexin V | P value (N = 5) | |
| No cytokine | 48 ± 9 | 90 ± 3 | ||
| IL-6 | 21 ± 7 | .008 (compared to No cytokine) | 56 ± 4 | .0003 (compared to No cytokine) |
| Osteoclasts | 16 ± 3 | .01 (compared to No cytokine) | 41 ± 9 | .01 (compared to No cytokine) |
| Osteoclasts + B-E8 | 25 ± 2 | .01 (compared to Osteoclasts) | 51 ± 8 | .05 (compared to Osteoclasts) |
| Osteoclasts + TACI-Fc | 29 ± 5 | .02 (compared to Osteoclasts) | 67 ± 8 | .01 (compared to Osteoclasts) |
| Osteoclasts + anti-IGF1R | 84 ± 8 | .0005 (compared to Osteoclasts) | 76 ± 9 | .003 (compared to Osteoclasts) |
| Osteoclasts + anti-IL-10 | 18 ± 2 | NS (compared to Osteoclasts) | 42 ± 4 | NS (compared to Osteoclasts) |
| Osteoclasts + anti-CCR2 | 19 ± 2 | NS (compared to Osteoclasts) | 43 ± 3 | NS (compared to Osteoclasts) |
*
NS: not significantly different