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. 1989 May;8(5):1455–1460. doi: 10.1002/j.1460-2075.1989.tb03528.x

Transcription factors AP-3 and AP-2 interact with the SV40 enhancer in a mutually exclusive manner.

F Mercurio 1, M Karin 1
PMCID: PMC400974  PMID: 2548845

Abstract

The 'core' sequence is critical for efficient transcriptional activity of the SV40 enhancer. Moreover, the core was shown to be involved in a signal transduction pathway elicited by treatment of cells with phorbol ester tumor promoters. We report here the identification and characterization of activator protein-3 (AP-3), which recognizes the core element. AP-3 was purified to near homogeneity and identified as a 48K polypeptide. The purified protein is an efficient transcriptional activator in vitro. In addition, we show that AP-3 and a second factor that recognizes the SV40 enhancer, AP-2, interact in a mutually exclusive manner. These studies should facilitate understanding of the mechanism by which the SV40 enhancer achieves its characteristic broad cell-type specificity.

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Selected References

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