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. 1989 Jul;8(7):1981–1986. doi: 10.1002/j.1460-2075.1989.tb03604.x

The cloned human oestrogen receptor contains a mutation which alters its hormone binding properties.

L Tora 1, A Mullick 1, D Metzger 1, M Ponglikitmongkol 1, I Park 1, P Chambon 1
PMCID: PMC401066  PMID: 2792078

Abstract

We demonstrate here that the human oestrogen receptor (hER) cDNA clone pOR8 obtained from MCF-7 cells contains an artefactual point mutation which results in the substitution of a valine for a glycine at amino acid position 400 (Gly-400----Val-400). This mutation in the hormone binding domain of the cloned hER destabilizes its structure and decreases its apparent affinity for oestradiol at 25 degrees C, but not at 4 degrees C, when compared with the wild-type hER with a Gly-400.

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Selected References

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