TABLE 2 .
Activities of the phuR and phuS promoters originating with different clinical isolates of P. aeruginosaa
| Strain | Promoter | Origin of promoter | Allele | Mean luminescence (± SD) | Fold change | P value |
|---|---|---|---|---|---|---|
| PAO1 | phuR | PAO1 | WT | 365 (±1,018) | 1 | |
| PAO1 | phuR | DK2-CF66-2008 | M1 | 34,111 (±3,379) | 93 | 0.00021 |
| PAO1 | phuR | DK2-CF173-2005 | M2 | 40,726 (±3,422) | 112 | 0.00004 |
| PAO1 | phuR | DK2-CF173-2002 | M3 | 1,879 (±3,422) | 5 | 0.16 |
| PAO1 | phuR | DK2-CF240-2002 | M4 | 7,584 (±496) | 21 | 0.00038 |
| PAO1 | phuR | DK2-CF222-2001 | M5 | 8,968 (±610) | 25 | 0.00023 |
| PAO1 | phuR | DK2-CF180-2002 | M6 | 6,723 (±701) | 18 | 0.00088 |
| PAO1 | phuR | DK1-P28F1-1992 | M8 | 13,329 (±1,482) | 37 | 0.00024 |
| PAO1 | phuR | DK1-P28F1-2009 | M9 | 12,205 (±603) | 33 | 0.00007 |
| PAO1 | phuR | DK1-CF30-2011 | M10 | 9,563 (±1,586) | 26 | 0.0011 |
| PAO1 | phuS | PAO1 | WT | 7,444 (±1,777) | 1 | |
| PAO1 | phuS | DK2-CF173-2005 | M2 | 12,030 (±3,191) | 1.6 | 0.01 |
a
Luminescence production from laboratory reference strain PAO1 (37) with phuR::lux reporter fusions was measured at exponential growth (OD600 = 0.15) in Luria-Bertani (LB) medium and normalized for differences in cell density. Mean luminescence production and standard deviations (SD) were calculated for three biological replicates. Statistical analysis concerning the difference between two means was done using a Student t test, and the P values denote the probability of the mutated alleles having expression equal to that of the wild type (WT).