Effect of combined naltrexone and bupropion therapy on the brain's reactivity to food cues

Abstract

Objective:

The significant weight loss observed with combination naltrexone-sustained release (SR) 32 mg and bupropion SR 360 mg (NB32) therapy is thought to be due, in part, to bupropion stimulation of hypothalamic pro-opiomelanocortin (POMC) neurons, and naltrexone blockade of opioid receptor-mediated POMC autoinhibition, but the neurobiological mechanisms are not fully understood. We assessed changes in brain reactivity to food cues before and after NB32 treatment.

Methods:

Forty women (31.1±8.1 years; body mass index: 32.5±3.9) received 4 weeks of NB32 or placebo, and were instructed to maintain their dietary and exercise habits. Functional magnetic resonance imaging responses (analyzed using SPM2 and clusters (>100 pixels)) to a 5-min food video (preparation of the subject's favorite food) and a 5-min neutral video (manipulation of neutral objects) under conditions of mild food deprivation (∼14 h) were assessed before and after treatment.

Results:

The food cues video induced positive brain activation in visual and prefrontal cortices, insula and subcortical brain regions. The group-by-treatment interaction on regional brain activation was significant and showed that whereas NB32 attenuated the activation in the hypothalamus in response to food cues (P<0.01), it enhanced activation in regions involved in inhibitory control (anterior cingulate), internal awareness (superior frontal, insula, superior parietal) and memory (hippocampal) regions (whole-brain analysis; P<0.05).

Conclusions:

Blunting the hypothalamic reactivity to food cues while enhancing the activation of regions involved with self-control and internal awareness by NB32 might underlie its therapeutic benefits in obesity.

Introduction

Although a number of signals that regulate food intake originate from internal sources that monitor the metabolic state of the body (that is, leptin, insulin, ghrelin and peptide YY), variables other than nutritional needs also profoundly influence food intake. These include pleasurable sensory responses from food, emotional variables and environmental factors.¹ Disruption in the sensitivity of the brain to these non-nutritional-related variables could result in excessive eating and obesity. Of particular relevance are the rewarding and conditioned responses triggered by palatable foods. Functional magnetic resonance imaging (fMRI) studies using blood oxygen level-dependent (BOLD) method showed that obese subjects activated brain regions related to motivation (dorsal striatum), salience attribution (orbitofrontal cortex) and taste information processing (insula) while viewing pictures of high-caloric food, which act as powerful food cues that generate food craving.^{2, 3} Imaging studies have also reported that obese subjects show greater disinhibition, which is associated with decreased activation in the anterior cingulate gyrus when exposed to food cues.⁴

Combination naltrexone-sustained release (SR) 32 mg and bupropion SR 360 mg (NB32) is a fixed dose drug combination under investigation as a treatment for obesity.⁵ Bupropion is approved for marketing in the United States for depression and smoking cessation. Functionally, bupropion is thought to increase the level of dopamine (DA) activity at specific brain regions, which appears to lead to a reduction in appetite and increase in energy expenditure. Bupropion is used in the treatment of depression not only for its clinical efficacy but also because of its side effect profile, which includes modest weight loss.⁶ Bupropion is also used as a treatment for smoking cessation, and ongoing trials are evaluating its utility to treat other types of drug addictions.⁷ Naltrexone is approved in the United States for the treatment of opioid addiction and for the treatment of alcoholism. Naltrexone works by blocking opioid receptors in the brain and inhibits the reinforcing aspects of addictive substances, reducing their perceived reward.⁸ Naltrexone might also decrease reward sensitivity to natural reinforcers as shown by reports of reduced reward to sweet-tasting foods in opioid addicts treated with naltrexone.⁹ Moreover, the combination of naltrexone and bupropion has been demonstrated to result in greater weight loss compared with either agent alone.⁵ The neurobiological mechanisms underlying the weight loss effects of NB32 are not fully understood but are thought to relate to effects on the reward system as well as bupropion stimulation of hypothalamic pro-opiomelanocortin (POMC) neurons, and naltrexone blockade of opioid receptor-mediated POMC auto-inhibition, with downstream effects to reduce food intake and increase energy expenditure. It may involve hypothalamic and brain stem mechanisms in which synergistic effects with the drug combination on food consumption have been reported.¹⁰ The present study assessed changes in brain reactivity to food cues before and after NB32 treatment using fMRI. We hypothesized that NB32 would decrease the reactivity of brain regions involved with reward while decreasing the deactivation of regions involved with regulatory control, relative to placebo.

Materials and methods

Subjects

Before study initiation, participants provided written informed consent approved by the Stony Brook University's Committee on Research Involving Human Subjects. Subjects were screened carefully with a detailed medical history, physical and neurological examination and urine toxicology for psychotropic drugs to ensure they were healthy at the time of the study and that they were not abusing drugs. Subjects (n=46) were included in the study if they were women, right-handed, 18–45 years old, healthy, able to understand and give informed consent and had 27⩽body mass index (BMI)⩽40 kg m⁻². Female subjects were used to minimize variability, because the vast majority of individuals who use pharmacotherapy for obesity are women. Exclusion criteria included obesity of known endocrine or genetic origin; history or presence of hepatic, renal, cardiovascular or gastrointestinal diseases; type 1 or type 2 diabetes mellitus requiring pharmacotherapy; serious psychiatric illness; bulimia or anorexia nervosa; history of alcohol or drug abuse or dependence (including nicotine); positive urine pregnancy test; head trauma with loss of consciousness >5 min; and any medical condition that may alter cerebral function or contraindications for magnetic resonance imaging (MRI). Subjects were asked to have their last meal completed by 1900 hours the evening before the day of the imaging visits and were scanned between 15 and 17 h after their last meal. Subjects were informed that blood sugar levels would be checked during the study to help ensure that they refrained from eating.

Study design

Subjects had two imaging visits, one at baseline and one after 4 weeks of treatment with NB32 or placebo. After the baseline visit, half of the participants were randomly assigned to the NB32 group and the other half were assigned to the placebo group. During the imaging visits, subjects underwent fMRI scans with the food video (FV) paradigm under fasting conditions. Study medication was withheld on the days of the scans until after completion of fMRI scans. The subjects were instructed to maintain their usual eating and exercise habits throughout the study to minimize the impact of changes in nutritional status or body weight on the brain activity.

NB32 and placebo administration

The naltrexone/bupropion combination evaluated in this study consisted of daily doses of NB32, combined in a trilayer tablet (each containing naltrexone SR 8 mg, bupropion SR 90 mg and an inert layer between). Study drugs were escalated to full dose over 3 weeks as follows: one tablet in the morning for 7 days→ one tablet in the morning and one tablet in the evening for 7 days→ two tablets in morning and one tablet in evening for following 7 days→ two tablets twice a day thereafter. Active and placebo tablets were blue, round and identical in appearance.

Food video paradigm

The subjects underwent an fMRI session at the baseline visit and at the week 4 visit. The day before the baseline visit, subjects rated their food preferences on 47 available food items in a pictographic ‘a la carte menu', which corresponded to 47 different FV fragments that were rated from 0 (less preferred) to 10 (most preferred). These food rating (‘liking') scores were used to select the 10 most preferred food items and their corresponding FV fragments for each subject. Each of these 1-min-long high-resolution FV fragments show close views of serving and consumption of the food items that were recorded indoors and saved in audio video interleave format by professional video personnel at the Brookhaven National Laboratory. The preferred FV fragments were contrasted against two different 2.5-min ‘non-food' control video fragments, which include routine administrative/technical work as control items, which were recorded indoors with the same resolution and format by the video personnel. For this purpose, 10-min-long movies with blocked design were created taking into account the subject's preferences. Each movie was composed by a control video epoch (beginning), the five FV fragments (random order) and a control video (end) epoch (Figure 1a). These different movies were presented to the subjects on MRI-compatible goggles connected to a personal computer during fasting condition. Image acquisition was performed continuously during the 10-min duration of the movie. The display software was written in Visual Basic and C languages and synchronized precisely with the MRI acquisition using a trigger pulse.

Figure 1.

fMRI paradigm. (a) Example of the timeline of the food-video stimulation. Each 600 seconds video contained 150 seconds control video (CV) fragments at its beginning and end, as well as 5-60 second food video (FV) fragments showing serving and consumption of the subject's favorite food items. (b) The general lineal model implemented in SPM2 was based on a castle design with 5 regressors modeling the FV epochs, which contrasted the FV fragments against the CV fragments.

MRI data acquisition

Subjects underwent MRI in a 4-Tesla whole-body Varian (Palo Alto, CA, USA)/Siemens (Erlangen, Germany) MRI scanner. A T2*-weighted single-shot gradient-echo planar imaging pulse sequence (echo time/repetition time (TE/TR)=20/1600 ms, 4-mm slice thickness, 1-mm gap, 35 coronal slices, 64 × 64 matrix size, 3.125 × 3.125 mm² in-plane resolution, 90° flip angle, 375 time points, 200.00 kHz bandwidth) with ramp-sampling and whole-brain coverage was used to collect functional images with BOLD contrast. Padding was used to minimize motion. Subject's motion was monitored immediately after each fMRI run using a k-space motion detection algorithm¹¹ written in Interactive Data Language (ITT Visual Information Solutions, Boulder, CO, USA). Earplugs (−28 dB sound pressure level attenuation; Aearo Ear TaperFit 2; Aearo Company, Indianapolis, IN, USA), headphones (−30 dB sound pressure level attenuation; Commander XG MRI Audio System, Resonance Technology Inc., Northridge, CA, USA) and a ‘quiet' acquisition approach were used to minimize the interference effect of scanner noise during fMRI.¹² Anatomical images were collected using a T1-weighted three-dimensional modified driven equilibrium Fourier transform pulse sequence¹³ (TE/TR=7/15 ms, 0.94 × 0.94 × 1.00 mm³ spatial resolution, axial orientation, 256 readout and 192 × 96 phase-encoding steps, 16 min scan time) and a modified T2-weighted hyperecho sequence¹⁴ (TE/TR=0.042/10 s, echo train length=16, 256 × 256 matrix size, 30 coronal slices, 0.86 × 0.86 mm² in-plane resolution, 5 mm thickness, no gap, 2 min scan time), and were reviewed by a neurologist to rule out gross morphological abnormalities of the brain.

fMRI analysis

Image reconstruction was performed using an iterative phase correction method in Interactive Data Language that minimizes signal loss artifacts in echo planar imaging.¹⁵ The first four imaging time points were discarded to avoid nonequilibrium effects in the fMRI signal. The statistical parametric mapping package SPM2 (Wellcome Trust Centre for Neuroimaging, London, UK) was used for subsequent analyses. A 4th degree B-spline function without weighting and without warping was used for image realignment (head motion was <2-mm translations and 2° rotations for all scans for all fMRI runs). Spatial normalization to the stereotactic space of the Montreal Neurological Institute was performed using a 12-parameter affine transformation with medium regularization, 16-nonlinear iterations and voxel size of 3 × 3 × 3 mm³ and the standard SPM2 echo planar imaging template. Spatial smoothing was carried out using an 8-mm full-width-half-maximum Gaussian kernel. BOLD–fMRI responses during the FV stimulation paradigm were estimated using a general linear model¹⁶ and a castle design matrix with five different FV regressors (Figure 1b), corresponding to the five FV fragments, convolved with low-pass (hemodynamic response function) and high-pass (cutoff frequency: 1/1200 Hz) filters. Thus, five contrast maps reflecting the % BOLD–fMRI signal change from baseline were obtained in fasting conditions and five in satiated conditions for each subject.

Statistical analyses

The BOLD–fMRI signals were included in a one-way analysis of variance (ANCOVA) model in SPM2 with two covariates: a zero-mean regressor reflecting the age of the participants and a zero-mean regressor reflecting the subjects' BMI. Brain activation clusters were corrected for multiple comparisons using the continuous random field calculation implemented in SPM2. The statistical significance for group analyses of the brain activation was based on a family-wise error (FWE) threshold P_corr<0.05, corrected for multiple comparisons at the voxel level, and a minimum cluster size of 20 voxels. The ANCOVA model included the terms for treatment and the appropriate baseline measurement. Type 3 sum of squares for the least square mean was used for the statistical comparison with 95% confidence interval also reported.

Functional ROI analyses

Brain activation clusters were further evaluated with region-of-interest (ROI) analyses to identify potential outliers and to report average values in a volume comparable to the image smoothness (for example, resolution elements or ‘resels')¹⁷ rather than single-voxel peak values. The volume of the resels was estimated using the random field calculation in SPM2 as a near cubic volume with Cartesian full-width-half-maximum=12.7 × 12.4 × 13.5 mm³. Thus, 9-mm isotropic masks containing 27 imaging voxels (0.73 ml) were defined at the centers of relevant activation clusters to extract the average %BOLD signal from individual contrast maps. These masks were created and centered at the precise coordinates listed in Table 2; the coordinates of the ROI masks were kept fixed across subjects and conditions. In addition to these functional ROIs, we analyzed the BOLD signals in the hypothalamus with anatomical 9-mm cubic ROI centered at Montreal Neurological Institute coordinates (x, y, z)=(0, −3, −6) mm to test for effects of the drug combination on reactivity to food cues in the hypothalamus. The average and s.d. values of BOLD signals within these ROIs were computed for each subject and fMRI run using a custom program written in Interactive Data Language.

Results

Forty-six non-smoking and right-handed women were recruited for the study. Three subjects withdrew from each treatment group (NB32: all because of adverse events (vomiting, headache and depression); placebo: one adverse event (migraine), one lost to follow-up and one failure to comply with protocol). Data from the 40 subjects who adhered to the treatment protocol and completed the fMRI studies are reported here. At baseline, the NB32 group and the placebo group were similar in age and BMI (Table 1). Body weight was not changed in the NB32 group or in the placebo group after 1 month of treatment (Table 1).

Table 1. Baseline characteristics of study subjects and weight over time.

Baseline characteristics
	NB32		Placebo
	Mean±s.d.	Range	Mean±s.d.	Range
Age (years)	30.9±7.8	20–44	31.4±8.5	19–45
BMI (kg m−2)	33.0±4.6	27.7–40.4	32.0±3.2	27.3–37.6
Weight over time
	NB32	Placebo	Placebo-corrected difference	P-value
Weight
Baseline (kg)	87.9±17.0	90.3±14.2	NA	NA
% Change at week 4	−0.99±0.44	−0.43±0.44	−0.56	0.38

Abbreviations: BMI, body mass index; NA, not applicable; NB32, naltrexone-sustained release (SR) 32 mg and bupropion SR 360 mg.

Baseline values are mean±s.d. Changes are least square mean±s.e.

N=20 for both NB32 and placebo groups.

Brain activation

The FV paradigm caused positive BOLD–fMRI responses (from control video epochs to FV epochs) in visual and prefrontal cortices, insula and subcortical brain regions (cerebellum, thalamus and hippocampus) separately for the placebo and NB32 groups and for baseline and treatment (P_FWE<0.05, ANCOVA; Figure 2 and Figure 3). At baseline, the NB32 group did not show significant activation in hippocampus, superior parietal cortex and posterior insula, whereas the placebo group showed activation in these regions (P_FWE<0.05). However, brain activation differences between the placebo and NB32 groups at baseline were not statistically significant (Table 2). The NB32 group had higher activation in anterior, middle and posterior cingulum, superior frontal and middle temporal cortices, superior parietal cortex and posterior insula after 4-week treatment than that at baseline (Table 2). Conversely, the placebo group had lower activation in anterior, middle and posterior cingulum, superior frontal and superior parietal cortices, hippocampus and parahippocampus after 4-week treatment than that at baseline (Table 2). There was a group-by-treatment interaction on brain activation in the superior frontal (Brodmann area: BA32), dorsal anterior cingulate (BA32), posterior insula, superior parietal (BA5) and hippocampal regions. These group-by-medication interaction effects were statistically significant (P_FWE<0.05; Table 2).

Figure 2.

The effect of NB32 and placebo on brain activity during food cue stimulation. Significant activated clusters during FV stimulation. SPG, superior parietal gyrus; ACG, anterior cingulate gyrus; PCG, posterior cingulate gyrus; IFG, inferior frontal gyrus; THL, thalamus; INS, insula; MB, midbrain; OCC, occipital cortex; HIP, hippocampus; CBL, cerebellum.

Figure 3.

The greater effect of NB32 as compared with placebo in response to visual food cues.

Table 2. Spatial coordinates of significant activated clusters during food video stimulation in the MNI stereotactic space (P<0.05; t-tests). Average t-score values in isotropic cubic regions of interest (27 voxels; 0.73 cc) centered at the (x, y, z) coordinates.

Region		Coordinates			t-score
		mm			PL			NB			PL>NB	NB>PL	NB>PL
	BA	x	y	z	BL	Tx	Tx>BL	BL	Tx	Tx>BL	BL	Tx	(Tx>BL)
Superior frontal	32	−12	39	39	NS	NS	−3.3	NS	3.7	NS	NS	3.8	4.2
Anterior cingulate	32	−9	30	33	NS	NS	−4.7	NS	3.8	NS	NS	4.1	4.8
Hippocampus	20	36	−27	−12	4.4	NS	NS	NS	4.7	4.4	NS	3.4	4.0
Hippocampus	20	33	−6	−27	NS	NS	−6.0	NS	NS	NS	NS	NS	3.4
Superior parietal	5	18	−45	66	3.2	−3.6	NS	NS	NS	3.2	NS	3.8	4.4
Posterior insula	48	42	−30	18	3.6	NS	NS	NS	NS	3.6	NS	3.1	4.0

Abbreviations: BA, Brodmann area; BL, baseline (food cue>neutral cue); MNI, Montreal Neurological Institute; NB, NB32 group; NB>PL(Tx>BL), group × treatment interaction; NS, not significant; PL, placebo group; Tx, after 4-week treatment (food cue>neutral cue). Tx>BL, treatment interaction.

N=20 for both the NB32 and placebo groups.

The BOLD–fMRI responses in the anatomical ROI in the hypothalamus did not show significant group differences at baseline. Hypothalamic activation after 4-week treatment was lower than that at baseline for the NB32 group (P<0.002) but there were no significant differences in hypothalamic activation in the placebo group (P>0.4). The group-by-medication interaction effect in the hypothalamus was also significant (P<0.01).

Discussion

Contrary to our initial hypothesis, participants treated with NB32 had enhanced activation in superior parietal (BA5), posterior insular, superior frontal (BA32), dorsal anterior cingulate (BA32), hippocampal regions and decreased activation in hypothalamus while viewing FVs as compared with placebo-treated participants under fasted conditions. We did not observe changes in brain regions such as nucleus accumbens and amygdala, which have been reported to become activated during imaging studies in response to food cues. It is possible that the food cue paradigm used in this study was not sufficient to induce activation in these areas.

The NB32-treated subjects, when compared with subjects who received placebo, showed increased activation in the superior frontal gyrus (BA32) and dorsal anterior cingulate (BA32) when exposed to the food cues. The superior frontal gyrus is associated with self-awareness, which is involved in introspection and linked to sensory perception. Introspection is a very high-level cognitive task that may include increases in attentional, memory and cognitive demands. When the brain diverts its resources to do challenging tasks, regions related to self-awareness are not engaged during sensory perception and can be suppressed.¹⁸ In this study, the decreased activation or no activation to the food cues in the superior frontal gyrus during baseline condition and after the placebo treatment would be consistent with the suppression of introspection. As the treatment with naltrexone reduces sensory perception of food cues,¹⁹ the enhanced reactivity of the superior frontal gyrus in NB32-treated group would be suggestive of an enhancement in self-awareness.

The anterior cingulate regulates both cognitive (dorsal cingulate) and emotional processing (ventral cingulate). The dorsal cingulate is associated with response conflict, decision-making and self-control. Using positron emission tomography to measure regional brain glucose metabolism in healthy controls (BMI range, 19–37 kg m⁻²) during baseline (no stimulation), we showed a negative correlation between BMI and metabolic activity in the prefrontal cortex and cingulate gyrus (BA 32).²⁰ Moreover, baseline metabolism in the prefrontal cortex and cingulate gyrus was positively associated with performance on tests of memory and executive function. These findings suggest that excessive weight might impair the activity of the prefrontal cortex and cingulate. In obese subjects, decreased metabolism in the prefrontal cortex and cingulate, which was associated with low levels of striatal DA D2 receptors,²¹ might contribute to excessive food intake by interfering with self-regulation, thus favoring impulsive and compulsive food intake. Thus, the enhanced reactivity of the dorsal cingulate in subjects that received NB32 treatment during exposure to food cues would be consistent with an enhancement in self-control. Indeed, the therapeutic effects of bupropion in smoking cessation are believed to be partly through enhancement of the anterior cingulate activation with concomitant improvement in the ability to resist craving.²² These reports mirror the results of the current study in which elevated baseline dorsal anterior cingulate activity was found in NB32-treated subjects. As bupropion increases DA and norepinephrine,²³ and both norepinephrine cells originating in the locus ceruleus and DA cells originating in the ventral tegmental area innervate the ventral anterior cingulate directly,²⁴ the enhanced activation of the cingulate could reflect increases in catecholamine signaling.

Subjects treated with NB32 also showed an enhanced response in the hippocampus, which is a brain region connected to the hypothalamus and insula. Preclinical studies show that hippocampal damage can result in hyperphagia.²⁵ The hippocampus modulates DA release in the ventral striatum, which is a mechanism by which the saliency of a stimulus is modulated.²⁶ It also regulates activity in prefrontal regions involved with inhibitory control.²⁷ Imaging studies have shown that obese and previously obese individuals have decreased responses in posterior hippocampus when tasting a liquid meal when compared with lean subjects. Persistence of an abnormal hippocampal response in the previously obese was associated with their susceptibility to relapse.²⁸ In two independent fMRI studies one of which evaluated activation responses to an implantable gastric stimulator developed for the treatment of obesity and the other measured the activation responses to balloon gastric distention, we showed activation of the hippocampus presumably from downstream stimulation of the vagus nerve and the solitary nucleus.^{29, 30} In these studies, we found the activation in the hippocampus was associated with a sensation of fullness. Thus, enhanced reactivity of the hippocampus by NB32 could also help interfere with excessive food intake.

Subjects treated with NB32 also showed enhanced brain activity in the insula, in addition to the enhanced response in the superior frontal and anterior cingulate regions. These regions appear to have a critical role in the initiation, maintenance, and adjustment of attentional control³¹ and in interoception.^{18, 32} Interoception refers to the monitoring of internal bodily states to maintain or procure homeostasis, possibly by rousing the organism through affective, motivational and attentional mechanisms. The insula is organized into multiple regions along a posterior-to-anterior gradient.³³ The posterior insula has been related to primary interoceptive operations, which is connected with primary and secondary somatosensory cortices and receives inputs from the hypothalamus, the amygdala and the limbic system.³⁴ Somatic and visceral sensory processing, taste perception and food cues activate the posterior insula.³³ The signals are then sent to the anterior insula, which is associated with affective and cognitive processes. Imaging studies showed hunger is associated with an increase in regional cerebral blood flow in the mid-posterior insular cortex, which attenuates with satiation.³⁵ Similarly, using fMRI we showed that distention of the stomach (using an inflatable balloon to mimic the gastric distension that occurs with food intake) activated the posterior insula, which most likely reflected its role in the awareness of body states.²⁹ Interestingly, obese individuals showed decreased cerebral blood flow³⁵ and decreased functional connectivity strength³⁶ in the posterior insula when they were hungry, suggesting impairment in interoceptive awareness. Thus, overeating in obese subjects could reflect in part an impaired awareness of internal hunger/satiety states secondary to impaired function of the posterior insula. Naltrexone, which reduces food intake in animal models of obesity and binge eating,³⁷ has been shown to modulate insular and dorsal striatal activity during food-related cues.¹⁹ Thus, the reported loss of body weight with treatment with NB32^{5, 10} could also relate to the greater reactivity of the posterior insula and a concomitant enhanced awareness and attention to interoceptive signals that reflect satiety (that is, fullness).

Finally, subjects treated with NB32 also showed an enhanced response in the superior parietal cortex (BA 5), which is a brain region involved with somatosensory processing,³⁸ spatial attention and conscious pain perception.³⁹ The activation of BA5 after NB32 suggests increased attention to the processing of the food cue experiment.

The enhanced regions observed in the current study (anterior cingulate, frontal cortex and hippocampus) overlap with regions of relatively decreased response to a Simon spatial incompatibility task in individuals with bulimia nervosa.⁴⁰ Women with bulimia nervosa have less self-regulatory control and respond more impulsively and make more errors to the task than healthy subjects. These tasks usually need greater engagement from frontostriatal regions. The enhanced activation of these regions after NB32 could reflect a functional enhancement of self-regulatory control processes in response to food cues.

Activation in the hypothalamus to food cues during fasting condition has been previously been demonstrated in fMRI studies.^{41, 42, 43} The hypothalamus activation was attenuated when the lean subjects were overfed,⁴³ but not in overweight and obese individuals,⁴¹ nor in obese subjects who have lost weight.⁴² A separate fMRI study demonstrated a delayed hypothalamic response after glucose ingestion in obese subjects.⁴⁴ Taken together, these studies suggest that obese subjects may have sluggish or absent homeostatic responses to satiety in the hypothalamus. In a post hoc analysis, we found that after NB32 treatment, subjects displayed attenuated activation in the hypothalamus in response to food cues. This overall attenuation of the hypothalamic response to food cues presumably occurs in juxtaposition to the enhanced activation of a subset of hypothalamic neurons (the POMC neurons), and reveals the complexity of the hypothalamus as an integrator of hunger and satiety signals. Interestingly, overall attenuated hypothalamic activity to food cues has also been observed following treatment with sibutramine, a predominantly serotonin and norepinephrine reuptake inhibitor thought to operate through a distinct mechanism of action compared with naltrexone/bupropion.⁴¹

It is noteworthy that little weight loss was observed in the NB32 subjects in this study. The study was designed to be of short duration and subjects were instructed to maintain their typical diet and exercise to minimize weight loss and the associated metabolic changes that could influence regional brain activity and confound the direct pharmacological effects of NB32 on brain. Thus, the changes in BOLD signal reported are more likely to be related to NB32 treatment than to any potential compensatory effects of weight loss.

Limitations of this study are: (1) when the subjects were retested 1 month after the treatment (placebo or drug), they showed decreased activation in response to the FV stimulation paradigm (Table 2). Decreased response to repeated stimulation paradigms (that is, semantic memory, working memory and visual attention) have previously been reported in other fMRI studies. These studies suggest that practice/habituation effects can reduce brain activation for visual tasks that involve attention and memory. (2) Only female subjects were enrolled in this study. Although this is consistent with the population of most obesity pharmacotherapeutic studies, caution should be used in extending these findings to men. (3) We did not observe significant correlations between subjective appetitive responses and brain activation responses to food cues, which would have facilitated the interpretation of our findings.

Conclusion

In a fasted condition and compared with the placebo group, the NB32 group following exposure to food cues had decreased activation in the hypothalamus and enhanced activation in the dorsal anterior cingulate, superior frontal, posterior insula, hippocampal and superior parietal regions, which are brain regions involved in inhibitory control, internal awareness, memory/conditioning and somatosensory processing. These findings suggest that, in addition to hypothalamic mechanisms, NB32-induced weight loss may also be because of changes in cortical reactivity to food cues, particularly brain regions implicated in interoception, memory and self-control.

GJW reports having received lecture fees and research funding from Orexigen Therapeutics Inc.; ED was an employee of Orexigen Therapeutics Inc. during the study. The remaining authors declare no conflicts of interest.