Table 1.
Effect of acid dilution on protein hydrolysis activity of kiwifruit protease (100 mg KF) on 600 mg of FDM (freeze dried meat) measured by the percentage of ammoniacal nitrogen in the supernatant of the hydrolysates after 60 min incubation at 37°C.
| Concentration | Ammoniacal N | ||
|---|---|---|---|
| HCl:H2O | HCl | KF | Mean |
| (mL:mL) | (M) | (mg) | (%) |
| 20:00 | * 0.074 | Nil | 12.94 |
| 20:00 | 0.074 | 100 | 13.56 |
| 10:10 | 0.037 | 100 | 19.05 |
| 9:11 | 0.034 | 100 | 29.12 |
| 8:12 | 0.030 | 100 | 35.62 |
| 7:13 | 0.026 | 100 | 36.67 |
| 6:14 | 0.022 | 100 | 37.65 |
| 5:15 | 0.019 | 100 | 37.04 |
| 4:16 | 0.015 | 100 | 32.59 |
| 3:17 | 0.011 | 100 | 30.93 |
| 2.5:17.5 | 0.009 | 100 | 30.41 |
| 1.25:18.75 | 0.005 | 100 | 30.00 |
| 00:20 | Nil | 100 | 27.68 |
| 00:20 | Nil | Nil | 12.60 |
The mean difference of all ammoniacal N replicates was 0.15%; 95% CI of difference −0.12 to 0.41; * The AOAC standard in vitro gastric molar concentration for drug dissolution testing.