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. 1989 Aug;8(8):2373–2379. doi: 10.1002/j.1460-2075.1989.tb08366.x

The PROS-35 gene encodes the 35 kd protein subunit of Drosophila melanogaster proteasome.

C Haass 1, B Pesold-Hurt 1, G Multhaup 1, K Beyreuther 1, P M Kloetzel 1
PMCID: PMC401172  PMID: 2477245

Abstract

The proteasome is a multicatalytic proteinase complex composed of nonidentical protein subunits. We have isolated a cDNA clone encoding the 35 kd proteasome subunit of Drosophila melanogaster and propose the designation PROS-35 for the corresponding gene. The deduced amino acid sequence reveals a region of striking homology to a tyrosine phosphorylation site of viral and cellular proteins suggesting a potential regulatory function for the 35 kd subunit within the proteinase complex. Immunocytochemical experiments reveal a tissue-dependent differential distribution of the proteasome between the nucleus and cytoplasm. In addition developmental analysis shows that the proteasome is highly expressed in the CNS of stage-16 embryos and in cardia, ventriculus and ovaries of adult flies. These data suggest a tissue- and development-dependent distribution of the proteasome in D. melanogaster.

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