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. 1989 Aug;8(8):2381–2386. doi: 10.1002/j.1460-2075.1989.tb08367.x

The spatial and temporal expression pattern of sevenless is exclusively controlled by gene-internal elements.

K Basler 1, P Siegrist 1, E Hafen 1
PMCID: PMC401176  PMID: 2792089

Abstract

The sevenless gene controls the fate of a single photoreceptor cell type in the developing eye of Drosophila. Its RNA and protein accumulate transiently in a subpopulation of cells in the developing eye imaginal disc. We have used P-element transformation with different minigenes and marker gene constructs to identify cis-acting regulatory regions required for the complex sevenless expression pattern. Our results indicate that the upstream region of the sevenless gene is devoid of any detectable regulatory elements and that sequences located 3' to the transcription start site are sufficient to promote the sevenless expression pattern. These gene-internal sequences function in both orientations on heterologous promoters, also when placed at the 3' end of a lacZ reporter gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banerji J., Olson L., Schaffner W. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes. Cell. 1983 Jul;33(3):729–740. doi: 10.1016/0092-8674(83)90015-6. [DOI] [PubMed] [Google Scholar]
  2. Basler K., Hafen E. Control of photoreceptor cell fate by the sevenless protein requires a functional tyrosine kinase domain. Cell. 1988 Jul 29;54(3):299–311. doi: 10.1016/0092-8674(88)90193-6. [DOI] [PubMed] [Google Scholar]
  3. Basler K., Hafen E. Sevenless and Drosophila eye development: a tyrosine kinase controls cell fate. Trends Genet. 1988 Mar;4(3):74–79. doi: 10.1016/0168-9525(88)90044-3. [DOI] [PubMed] [Google Scholar]
  4. Basler K., Hafen E. Ubiquitous expression of sevenless: position-dependent specification of cell fate. Science. 1989 Feb 17;243(4893):931–934. doi: 10.1126/science.2493159. [DOI] [PubMed] [Google Scholar]
  5. Bienz M., Saari G., Tremml G., Müller J., Züst B., Lawrence P. A. Differential regulation of Ultrabithorax in two germ layers of Drosophila. Cell. 1988 May 20;53(4):567–576. doi: 10.1016/0092-8674(88)90573-9. [DOI] [PubMed] [Google Scholar]
  6. Bowtell D. D., Simon M. A., Rubin G. M. Nucleotide sequence and structure of the sevenless gene of Drosophila melanogaster. Genes Dev. 1988 Jun;2(6):620–634. doi: 10.1101/gad.2.6.620. [DOI] [PubMed] [Google Scholar]
  7. Bowtell D. D., Simon M. A., Rubin G. M. Ommatidia in the developing Drosophila eye require and can respond to sevenless for only a restricted period. Cell. 1989 Mar 24;56(6):931–936. doi: 10.1016/0092-8674(89)90626-0. [DOI] [PubMed] [Google Scholar]
  8. Dudler R., Travers A. A. Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies. Cell. 1984 Sep;38(2):391–398. doi: 10.1016/0092-8674(84)90494-x. [DOI] [PubMed] [Google Scholar]
  9. Grosschedl R., Baltimore D. Cell-type specificity of immunoglobulin gene expression is regulated by at least three DNA sequence elements. Cell. 1985 Jul;41(3):885–897. doi: 10.1016/s0092-8674(85)80069-6. [DOI] [PubMed] [Google Scholar]
  10. Hafen E., Basler K., Edstroem J. E., Rubin G. M. Sevenless, a cell-specific homeotic gene of Drosophila, encodes a putative transmembrane receptor with a tyrosine kinase domain. Science. 1987 Apr 3;236(4797):55–63. doi: 10.1126/science.2882603. [DOI] [PubMed] [Google Scholar]
  11. Hiromi Y., Kuroiwa A., Gehring W. J. Control elements of the Drosophila segmentation gene fushi tarazu. Cell. 1985 Dec;43(3 Pt 2):603–613. doi: 10.1016/0092-8674(85)90232-6. [DOI] [PubMed] [Google Scholar]
  12. Hultmark D., Klemenz R., Gehring W. J. Translational and transcriptional control elements in the untranslated leader of the heat-shock gene hsp22. Cell. 1986 Feb 14;44(3):429–438. doi: 10.1016/0092-8674(86)90464-2. [DOI] [PubMed] [Google Scholar]
  13. Klemenz R., Weber U., Gehring W. J. The white gene as a marker in a new P-element vector for gene transfer in Drosophila. Nucleic Acids Res. 1987 May 26;15(10):3947–3959. doi: 10.1093/nar/15.10.3947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mismer D., Rubin G. M. Analysis of the promoter of the ninaE opsin gene in Drosophila melanogaster. Genetics. 1987 Aug;116(4):565–578. doi: 10.1093/genetics/116.4.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. O'Hare K., Rubin G. M. Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell. 1983 Aug;34(1):25–35. doi: 10.1016/0092-8674(83)90133-2. [DOI] [PubMed] [Google Scholar]
  16. O'Kane C. J., Gehring W. J. Detection in situ of genomic regulatory elements in Drosophila. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9123–9127. doi: 10.1073/pnas.84.24.9123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ready D. F. A multifaceted approach to neural development. Trends Neurosci. 1989 Mar;12(3):102–110. doi: 10.1016/0166-2236(89)90166-5. [DOI] [PubMed] [Google Scholar]
  18. Riddihough G., Pelham H. R. Activation of the Drosophila hsp27 promoter by heat shock and by ecdysone involves independent and remote regulatory sequences. EMBO J. 1986 Jul;5(7):1653–1658. doi: 10.1002/j.1460-2075.1986.tb04408.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Robertson H. M., Preston C. R., Phillis R. W., Johnson-Schlitz D. M., Benz W. K., Engels W. R. A stable genomic source of P element transposase in Drosophila melanogaster. Genetics. 1988 Mar;118(3):461–470. doi: 10.1093/genetics/118.3.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schneuwly S., Klemenz R., Gehring W. J. Redesigning the body plan of Drosophila by ectopic expression of the homoeotic gene Antennapedia. 1987 Feb 26-Mar 4Nature. 325(6107):816–818. doi: 10.1038/325816a0. [DOI] [PubMed] [Google Scholar]
  21. Simon J. A., Sutton C. A., Lobell R. B., Glaser R. L., Lis J. T. Determinants of heat shock-induced chromosome puffing. Cell. 1985 Apr;40(4):805–817. doi: 10.1016/0092-8674(85)90340-x. [DOI] [PubMed] [Google Scholar]
  22. Smale S. T., Baltimore D. The "initiator" as a transcription control element. Cell. 1989 Apr 7;57(1):103–113. doi: 10.1016/0092-8674(89)90176-1. [DOI] [PubMed] [Google Scholar]
  23. Tomlinson A., Bowtell D. D., Hafen E., Rubin G. M. Localization of the sevenless protein, a putative receptor for positional information, in the eye imaginal disc of Drosophila. Cell. 1987 Oct 9;51(1):143–150. doi: 10.1016/0092-8674(87)90019-5. [DOI] [PubMed] [Google Scholar]

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