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Expression of sup35-25 from a high copy number plasmid increases the amounts of Sup35 and causes anti-suppression of lys2-87 mutation. Detection of Sup35 by protein gel blot in [isp−] strains transformed by pRSU3-25 and pRS425 (A); growth of transformants on SMM-Leu required for maintenance of plasmids and on SMM-Leu-Lys necessary for detection of lys2-87 manifestation (B).
