Figure 2.

Maternally GFP-tagged centriolar proteins are incorporated into the daughter centrioles of the GC and the PCL. (A and C) Immediately post-fertilization, the first two zygotic centrioles are not labeled by maternal centriolar proteins, but are labeled by Asl (magenta). (B and D) During the zygote’s first mitosis, an antibody against Asl labels all centrioles, but Sas-6-GFP (B) or Sas-4-GFP (D) labels only two centrioles. This indicates that three types of centrioles are observed: a GC, the presumed PCL, and two DCs.