Apocynin attenuates ventilator-induced lung injury in an isolated and perfused rat lung model

Abstract

Rationale

Apocynin suppresses the generation of reactive oxygen species (ROS) that are implicated in ventilator-induced lung injury (VILI). We thus hypothesized that apocynin attenuates VILI.

Methods

VILI was induced by mechanical ventilation with tidal volume (V_t) of 15 ml/kg in isolated and perfused rat lung. Apocynin was administered in the perfusate at onset of mechanical ventilation. A group ventilated with low V_t of 5 ml/kg served as control. Hemodynamics, lung injury indices, inflammatory responses, and activation of apoptotic pathways were determined upon completion of mechanical ventilation.

Results

There was an increase in lung permeability and lung weight gain after mechanical ventilation with high V_t, compared with low V_t. Levels of inflammatory cytokines including interleukin-1b (IL-1b), tumor necrosis factor-alpha (TNF-a), and macrophage inflammatory protein-2 (MIP-2) increased in lung lavage fluids; concentrations of carbonyl, thiobarbituric acid reactive substances, and H₂O₂ were higher in perfusates and lung lavage fluids, and expression of myeloperoxidase, JNK, p38, and caspase-3 in lung tissue was greater in the high-Vt than in the low-Vt group. Administration of apocynin attenuated these inflammatory responses and lung permeability associated with decreased activation of nuclear factor-κB.

Conclusions

VILI is associated with inflammatory responses including generation of ROS, cytokines, and activation of mitogen-activated protein kinase cascades. Administration of apocynin at onset of mechanical ventilation attenuates inflammatory responses and VILI in the isolated, perfused rat lung model.

Introduction

Inappropriate mechanical ventilation can initiate or exacerbate lung injury leading to ventilator-induced lung injury (VILI), as a result of volutrauma [1] and biotrauma [2]. Animal studies demonstrated that mechanical ventilation with high tidal volume (V_t) led to increased neutrophil infiltration [3] and production of proinflammatory cytokines in otherwise healthy lung [4], indicative of a link between volutrauma and biotrauma. In patients with acute respiratory distress syndrome (ARDS), application of low-V_t ventilatory strategies has been shown to decrease mortality [5]. However, low-V_t strategies may not meet the need in all patients, and additional pharmacological therapy may be required to target ongoing inflammatory responses in ARDS.

Lung epithelial cells have been shown to increase nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity in response to cyclic mechanical stretch resulting in production of superoxide (O₂⁻) [6]. Reactive oxygen species (ROS) can activate mitogen-activated protein kinase (MAPK) cascades and lead to excessive production of proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) [6–8] leading to epithelial drop off or cell death in the context of VILI [9]. In a mouse model [10], early increase in phosphorylation of p38 and extracellular signal regulated kinase (ERK) has been observed in response to mechanical ventilation with no significant change in activation of c-Jun NH2-terminal kinases (JNK). In isolated, nonperfused mouse lung [7], activation of p38, ERK, and JNK has been reported after mechanical ventilation. Taken together, the data suggest that NADPH oxidase may play an important role in the pathogenesis of VILI but may be overlooked as a therapeutic target in the lung research field.

Apocynin (4-hydroxy-3-methoxy-acetophenone), a naturally occurring methoxy-substituted catechol, inhibits NADPH oxidase in activated leukocytes, preventing generation of ROS [11]. Several in vivo studies have shown that apocynin can reduce neutrophil oxidative burst and neutrophil chemotaxis and thus attenuate neutrophil-mediated cell damage [12]. Apocynin has also been shown to decrease monocyte–endothelium interaction in vitro upon stimulation with TNF-α [13]. We hypothesized that apocynin attenuates VILI by inhibition of ROS generation and inflammatory responses.

Methods

Animal preparation

The study protocol was approved by the Institutional Board for Animal Care and Use. The in situ isolated, perfused lung model has been previously described [14]. Briefly, male Sprague–Dawley rats (SD rat) weighing 250–350 g were anesthetized with intraperitoneal injection of sodium pentobarbital. Tracheotomy was performed, and mechanical ventilation was applied (Rodent ventilator model 683; Harvard Apparatus, South Natick, MA, USA) at tidal volume (V_t) of 5 ml/kg and positive end-expiratory pressure (PEEP) of 2 cmH₂O. After sternotomy, heparin (1 unit/g) was injected into the right ventricle, through which pulmonary artery was catheterized. The left atrium was cannulated with catheter entering through apex of the heart. The pulmonary venous outflow was diverted into a reservoir. To prevent flow back into the ventricles, an additional ligation was performed above the atrioventricular junction. The lungs were perfused (Minipulse 2; Gilson Medical Electronic, Middleton, WI, USA) at constant flow of 30 μl/min/g body weight. Pulmonary artery pressure (P_pa) and pulmonary venous pressure (P_pv) were monitored. Rat weight was determined to reflect lung weight in the in situ system. Pulmonary arterial resistance (R_a) and venous resistance (R_v) were calculated using the following equations: R_a = (P_pa − P_pc)/Q, and R_v = (P_pc − P_pv)/Q, where Q is perfusate flow, P_pc is pulmonary capillary pressure.

Experimental protocols

The isolated lungs were randomly divided into three groups and ventilated for 2 h with either low V_t of 5 ml/kg (LVt), high V_t of 15 ml/kg (HV_t) or high V_t in combination with treatment by apocynin (HVt + Apo). PEEP of 2 cmH₂O was applied in all groups. Apocynin (Biomol, USA) was administered at 0.01, 0.1 or 0.2 mM/l in total volume of 40 ml circulating perfusate at onset of mechanical ventilation. Vascular permeability was determined by measuring pulmonary capillary filtration coefficient (K_fc) as previously described [15–17].

Myeloperoxidase assay

Concentration of myeloperoxidase (MPO), an index of neutrophil sequestration in the lungs, was measured as previously described [18].

Carbonyl and thiobarbituric acid reactive substances (TBARS) assay

Protein carbonyl content was measured by protein carbonyl assays (Geneteks Biosciences, Inc., San-Chong City, Taipei). TBARS level in serum was measured using OxiSelectTM TBARS assay kit (Geneteks Biosciences, Inc.).

H₂O₂ assay

Perfusate was centrifuged at 1,000 9 g within 30 min, the supernatant was collected, and 50 μl H2O2 reaction mix containing 46 μl assay buffer, 2 μl OxiRedTM probe solution, and 2 μl HRP solution (BioVision, USA) was added for incubation for 10 min. Absorbance was read at 570 nm (SpectraMax M5; Molecular Devices, USA). Concentration was calculated based on H₂O₂ standard curves.

Cytokines assays

Levels of interleukin-1β (IL-1β), TNF-α and macrophage inflammatory protein 2 (MIP-2) in lavage fluids were measured using commercial enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems, Oxon, UK). Absorbance was read at 450 nm (SpectraMax M5; Molecular Devices, USA).

Western blotting analysis

Lung tissues were homogenized using lysis buffer containing protease inhibitor cocktail (Roche, USA) and phosphatase inhibitor cocktail (Roche, USA). Total protein extracts were separated on 10% sodium dodecyl sulfate polyacrylamide gel, and electrotransferred onto polyvinylidine fluoride (PVDF) membrane (Millipore, USA). The membrane was blocked with 5% nonfat dry milk in Tris-buffered saline (TBS) containing 0.1% Tween 20 (TBST) for 1 h. Antibodies against phosphop44/42 MAPK (ERK1/2), phospho-p44/42 MAPK (ERK1/2), phospho-stress-activated protein kinases (SAPK)/JNK, phospho-p38 MAPK, anti-p44/42 MAPK (ERK1/2), anti-SAPK/JNK, and anti-p38 MAP kinase (1:1,000; Cell Signaling Technology, Beverly, MA, USA) were used. Antibodies against glyceraldehyde 3-phosphate dehydrogenase (GADPH, 1:10,000; Lab Frontier), JNK1 (1:1,000; Santa Cruz Biotechnology), and caspase- 3 (1:2,000; Cell Signaling Technology) were used. The appropriate secondary antibodies were used (1:10,000 horseradish peroxidase antirabbit; Jackson Immuno Research Laboratories). Visualization was performed by enhanced chemiluminescence (Visual Protein Biotechnology Corp., Taiwan). Protein bands were quantified using Kodak 1D image analysis (Eastman Kodak Company, Rochester, NY, USA).

Lung histopathology

Upon completion of experiments, the lungs were dissected and fixed immediately in 10% neutral buffered formalin. The right middle lobes were dehydrated through a graded series of alcohol, cleared in xylene, embedded in paraffin, and stained with hematoxylin/eosin. Three slides from each animal were evaluated for a total of six rats per group. Lung injury score was scaled from 1 to 4 based on our previous study [19]. The score includes perivascular edema 1, peribronchial edema 2, interstitial edema 2, alveolar edema 3, perivascular cell infiltration 2, interstitial cell infiltration 3, and alveolar cell infiltration 4. The lung injury score was evaluated by two pathologists who were blinded to the experimental conditions.

Immunohistochemistry

Lung slides coated with poly-L-lysine (Sigma, St. Louis, MO, USA) were deparaffinized and rehydrated using xylene and ethanol, and placed in 3% H₂O₂ for 15 min. The slides were incubated with 1:60 dilution of monoclonal nuclear factor kappa-light-chain-enhancer of activated B cells (NF)-κB (Cell Signaling Technology, Beverly, MA, USA), incubated at 4_C overnight, and stained with diaminobenzidine (DAB) (Dako, USA) and Mayer’s hematoxylin (Dako). Analysis was performed under Eclipse 80i microscope (Nikon, Japan) using Image Pro Plus 5.0 (Media Cybernetics, USA). Cells with positive nuclear NF-κB staining were counted out of a total of 100 cells in each slide from three animals in each group of LVt, HVt or HVt + Apo, respectively. Cell counting was performed by a pathologist who was blinded to the experimental conditions.

Statistical analysis

Systat10.0 (Systat Software Inc., San Jose, CA, USA) was used for statistical analysis. Comparisons among all groups were conducted using two-way analysis of variance (ANOVA) for repeated measurements. Comparison between baseline and post-VILI values within group was conducted using Student’s paired t test. Values are expressed as mean ± standard deviation (SD). P<0.05 was considered statistically significant.

Results

VILI model

There was no significant difference in hemodynamics among the groups at baseline and at the end of study (Supplementary Table 1).

Lung weight gain was higher in the HV_t than in the LV_t group, which was decreased in the presence of apocynin (Table 1). Lung weight gain was in agreement with increased lung K_fc in the HV_t group (Table 1). The increased permeability was further confirmed by the histological analysis showing perivascular edema and intra-alveolar hemorrhage in the HV_t group compared with the LV_t group. The apocynin-treated group showed decreased histological alterations (Fig. 1a–c) and lung injury score (Fig. 1d).

Table 1.

LWG and Kfc.

Group	N	LWG (g)	Kfc (cmH2O/min/mL)
			Baseline	After injury
Control	6	0.09 ± 0.06	0.17 ± 0.07	0.19 ± 0.07
VILI	6	1.37 ± 0.59*	0.20 ± 0.05	0.37 ± 0.04*
VILI + apocynin	6	0.79 ± 0.46*#	0.20 ± 0.04	0.24 ± 0.05#

Values are mean ± SD. LWG lung weigh gain, Kfc pulmonary capillary filtration coefficient.

^*p < 0.05 compared with control;

^#p< 0.05 compared with VILI.

Figure 1.

Apocynin improved lung histology. A. Animals received LV_t B. Animals were ventilated with HV_t. Lung histology was characterized by perivascular edema, interstitial and intra-alveolar leukocyte infiltration, and marked heterogeneity in alveolar inflation. C. Treatment with apocynin improved lung histology. D. Treatment with apocynin decreased lung injury score. E. Treatment with apocynin decreased MPO concentration in lung lavage fluids under mechanical ventilation with HVt. *p< 0.05 for HV_t versus LV_t; ⁺p < 0.05 for HV_t versus HV_t + Apo, at indentical condition, respectively.

Inflammatory responses

Concentration of MPO was measured to reflect pulmonary neutrophil infiltration. The level of MPO in lung lavage fluids increased in the HV_t group compared with the LV_t group (Fig. 1e). This is in agreement with the lung histology analysis showing interstitial and intraalveolar leukocytic infiltrates and proteinaceous exudates in the HVt group (Fig. 1b). Treatment with apocynin decreased concentration of MPO (Fig. 1e) and improved lung histology (Fig. 1c).

A maximal level of carbonyl, TBARS, and H₂O₂ was noted in perfusates (Supplementary Fig. 1A–C) and lung lavage fluids (Fig. 2a–c) 30 min after onset of mechanical ventilation, and their values were higher in the HV_t group than in the LV_t group. The levels of MIP-2, IL-1β, and TNF-α in lavage fluids were higher in the HV_t group than in the LV_t group (Supplementary Fig. 1D–F). Administration of apocynin largely attenuated the oxidative stress and cytokine responses in dose-dependent manner at doses of 0.01 and 0.1 mM/l (Fig. 2 and Supplementary Fig. 1). A higher dose of apocynin at 0.2 mM/l did not produce any further effects as compared with the dose used at 0.1 mM/l (Fig. 2 and Supplementary Fig. 1).

Figure 2.

Apocynin dose-dependently decreased oxidative stress. Concentrations of carbonyl, TBARS, and H₂O₂ were measured in lung lavage fluids (a–c) at the end of study.

MAPK, apoptotic, and NF-κB signaling pathways

Phosphorylation of JNK and P-38 but not ERK (data not shown) increased in lung tissue after high-Vt mechanical ventilation (MV) (Fig. 3a, b). Protein expression of apoptotic caspase-3 in the lung was higher in the HV_t than in the LV_t group (Fig. 3c). Administration of apocynin attenuated the MAPK and apoptotic signaling pathways (Fig. 3a–c).

Figure 3.

Apocynin attenuated activation of JNK, p-38 and caspase-3 expression in lung. Lung tissue was homogenized for Western blot by using appropriate antibodies.

Immune staining of nuclear NF-κB increased in lung tissue after mechanical ventilation with HV_t compared with LV_t (Fig. 4a, b). Administration of apocynin decreased the NF-κB activation seen in the HVt group (Fig. 4c). The quantitative data of cells with positive nuclear NF-κB staining are reported in Fig. 4d.

Figure 4.

Apocynin decreased NF-κB activation. Nuclear staining of NF-κB in lung tissue after mechanical ventilation with LVt (a) and HVt in the absence (b) and presence of apocynin (c), Arrows indicate NF-κB staining. High power (x100) in lower panel. d. Percentage of cells with positive nuclear staining for NF-κB.

Discussion

A major finding of the present study is that mechanical ventilation with high V_t increased generation of ROS, cytokine responses, and activation of MAPK and NF-κB associated with lung injury. Administration of apocynin depressed oxidative stress, attenuated inflammatory responses, and reduced VILI.

Our VILI model reproduces many features reported in other studies [1–4, 14], including inflammatory responses and structural lung damage. Our results are in agreement with those previously reported showing an inability of endogenous host defenses to increase antioxidant capacity, leading to failure of lung repair mechanism [20]. It is interesting that treatment with apocynin at onset of mechanical ventilation showed dramatic effects in attenuation of VILI. Our study suggests that the oxidative stress implicated in mechanical ventilation is a significant therapeutic target in the context of VILI.

Apocynin is a strong oxidative inhibitor that has been demonstrated to block NADPH oxidase in neutrophils [21, 22], macrophages [22, 23], and endothelium [24] through inhibition of p47phox translocation [25], without interfering with other immune biological functions of cell systems. This is an important therapeutic mechanism of apocynin, as NADPH plays a crucial role in the complex cell–cell interaction during oxidative status [26]. We demonstrate that treatment with the antioxidant apocynin dramatically attenuated inflammatory responses including cytokines and MAPK activation. Although it is difficult to know the exact timing for the generation of cytokines and ROS in the in vivo model, it has been demonstrated that cytokine stimulation leads to accumulation of ROS, which is essential for prolonged MAPK activation and cell death. In turn, ROS generation can stimulate host cytokine production [27]. Thus, the observed decrease in cytokine responses, and reduced activation of MAPK and NF-κB, could be interrelated after the treatment with apocynin.

Apocynin is not well studied as a therapeutic approach in the context of VILI despite the fact that apocynin has been used to decrease TNF-a production and to reduce pulmonary artery endothelial cell apoptosis in endotoxemic mice [28, 29]. Use of apocynin blocks MAPK signal transduction and attenuates apoptotic cell death by blocking intrinsic and extrinsic signaling pathways during ischemia and reperfusion injury [30–37]. Several studies [21, 38, 39] have shown organ protective effects of apocynin by inhibition of cyclooxygenase metabolites in a variety of animal models. Cyclooxygenase byproducts such as thromboxane B2 play an important role to increase pulmonary vascular permeability and to induce pulmonary hypertension. We believe that this is the first report showing beneficial effects of apocynin in the context of VILI.

There are a couple of limitations to the study. We employed an isolated perfused lung model to minimize any hemodynamic effects on lung injury, but the model does not provide interactions with other organ system. Although our approach is relevant clinically, as apocynin was administered at onset of mechanical ventilation to maximize protective effects, late treatment with apocynin is warranted for future studies, as VILI is long established in some clinical situations.

In summary, our results suggest that ROS play an important role in the context of VILI. Application of apocynin can effectively attenuate VILI by inhibition of inflammatory responses associated with depressed oxidative stress in the isolated, perfused lung model.