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. Author manuscript; available in PMC: 2014 May 20.
Published in final edited form as: Peptides. 2013 Mar 15;43:137–145. doi: 10.1016/j.peptides.2013.03.008

Fig. 4.

Fig. 4

Effect of pretreatment with VIP on desensitization of VPAC2-mediated adenylyl cyclase activity in gastric muscle from wild type mice and caveolin-1−/− mice. Adenylyl cyclase activity in response to VIP was measured as increase in cAMP formation in gastric muscle cells from wild type and caveolin-1−/− mice before and after treatment of the cells with VIP (1 μM) for 30 min. Desensitization of VPAC2 receptors was assessed by the decrease in cAMP response after treatment with VIP. Results are expressed as pmol of cAMP/mg protein. Pretreatment of cell with VIP inhibited cAMP formation (desensitization) in response to subsequent addition of VIP (1 μM). Desensitization of VPAC2 receptors was significantly attenuated in muscle cells from caveolin-1−/− mice. Values are expressed as means ± SE of 4 experiments.