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. Author manuscript; available in PMC: 2014 Jun 25.
Published in final edited form as: Biochemistry. 2013 Jun 14;52(25):4364–4372. doi: 10.1021/bi400342t

Figure 1. NO abrogates quinone-dependent PARP activation.

Figure 1

BAEC were exposed to Deta/NO (500 µM) for 1 h prior to treatment with menadione (20 µM) or DMNQ (20 µM) for an additional 4 h. (A) Protein poly(ADP-ribosyl)ation was measured by Western blotting using antibody against poly(ADP-ribose). (B) Densitometric analysis of protein PAR normalized to β-actin levels. NAD+ (C) and NADH (D) levels were measured by HPLC and normalized to total protein. Control (black bars), menadione-treated (white bars), and DMNQ-treated (grey bars) groups are shown. Values represent means ± SE; n = 3. * p < 0.05 compared to untreated control, ** p < 0.05 compared to samples without Deta/NO.