Figure 4. The PC synthetic rate is increased in the absence of increased phospholipid content in lungs from Ulk1/2 DKO and Atg5 KO mice. Lungs from (A) Ulk1/2 Ctrl; Ulk1/2 DKO at ED 18.5 (n = 7 for Ulk1/2 Ctrl and n = 3 for Ulk1/2 DKO) and P1 (n = 5 for Ulk1/2 Ctrl and Ulk1/2 DKO respectively) (B) Atg5 Ctrl and Atg5 KO at ED18.5 (n = 4 for Atg5 Ctrl and n = 3 for Atg5 KO) and P1 (n = 7 for Atg5 Ctrl and n = 4 for Atg5 KO) were harvested and incubated in vitro with medium containing ³H-choline followed by measurement of the incorporation of ³H choline into newly synthesized PC. Data are presented as the mean ± SEM * denotes P value < 0.05. (C) Total phospholipid content was measured at P1. Data are presented as mean ± SEM with n = 4 for each experimental group.