Decarboxylation of Pyruvate to Acetaldehyde for Ethanol Production by Hyperthermophiles

Abstract

Pyruvate decarboxylase (PDC encoded by pdc) is a thiamine pyrophosphate (TPP)-containing enzyme responsible for the conversion of pyruvate to acetaldehyde in many mesophilic organisms. However, no pdc/PDC homolog has yet been found in fully sequenced genomes and proteomes of hyper/thermophiles. The only PDC activity reported in hyperthermophiles was a bifunctional, TPP- and CoA-dependent pyruvate ferredoxin oxidoreductase (POR)/PDC enzyme from the hyperthermophilic archaeon Pyrococcus furiosus. Another enzyme known to be involved in catalysis of acetaldehyde production from pyruvate is CoA-acetylating acetaldehyde dehydrogenase (AcDH encoded by mhpF and adhE). Pyruvate is oxidized into acetyl-CoA by either POR or pyruvate formate lyase (PFL), and AcDH catalyzes the reduction of acetyl-CoA to acetaldehyde in mesophilic organisms. AcDH is present in some mesophilic (such as clostridia) and thermophilic bacteria (e.g., Geobacillus and Thermoanaerobacter). However, no AcDH gene or protein homologs could be found in the released genomes and proteomes of hyperthermophiles. Moreover, no such activity was detectable from the cell-free extracts of different hyperthermophiles under different assay conditions. In conclusion, no commonly-known PDCs was found in hyperthermophiles. Instead of the commonly-known PDC, it appears that at least one multifunctional enzyme is responsible for catalyzing the non-oxidative decarboxylation of pyruvate to acetaldehyde in hyperthermophiles.

1. Introduction

Thermophilic microorganisms can be categorized into several groups: moderate thermophiles, or simply thermophiles, are those that grow optimally between 50–64 °C, extreme thermophiles are those with optimal growth temperatures between 65–79 °C. Finally, the organisms that can grow optimally above 80 °C are called hyperthermophiles. Hyperthermophiles can survive at room temperature for long periods of time, but cannot propagate at temperatures lower than 50 °C [1,2,3,4,5].

Hyperthermophilic microorganisms are widely studied for their remarkable scientific values and industrial potential. It is generally accepted that hyperthermophilic enzymes have very similar functions and catalytic mechanisms to their mesophilic ones. However, most of the hyperthermophilic enzymes characterized so far have optimum temperatures close to the host organism’s growth requirements; thus, due to their intrinsic properties, the enzymes are stable and active under conditions that are detrimental to their mesophilic counterparts. Interestingly, enzymes from such extremophiles usually show increased stability not to one, but to several environmental factors. There are a number of advantages for using the hyper/thermophilic enzymes (especially for industrial applications) over their mesophilic partners, including the reduced risk of contamination during industrial processes, the possibility of self-distillation of the products at high temperatures, decreased viscosity and increased solubility/bioavailability of both the enzyme and the substrate(s) leading to minimization of the diffusion limitations, and elimination of the costly transportation under cold temperature-controlled environment [6,7,8,9,10].

The aforementioned properties along with high demand from the biotech industries for the development of “tailor-made” bio-catalysts have created significant attention on the biochemistry and physiology of these organisms. Enzymes such as proteases, polymerases, hydrolases, isomerases, lipases, and oxidases are studied for their potential biotechnological exploitation, with the ultimate goal of using them or their products (mainly enzymatic) for biotechnological applications.

It is also appealing to determine the molecular, biochemical, physiological, and evolutionary mechanisms that enable these organisms to adapt to such hostile environments. Furthermore, hyperthermophilic proteins serve as models to study enzyme evolution, structure-function relationships and catalytic mechanisms. The findings of these studies can benefit the design of highly stable and active enzymes to be used for many applications [6,11,12].

2. Microbial Production of Ethanol

Demand for biofuel as substitutes for oil-based fuels is increasing due to concerns related to national security, economic stability, environmental impacts, and global warming. The national research council of the United States has predicted that, by 2020, half of all organic chemicals and materials will be produced by bioconversion. Bio-ethanol can also be used as a precursor for many other commodity chemicals, such as acetaldehyde, acetic acid and their derivatives [13,14,15].

The most commonly used ethanologenic organisms being intensively studied or already in use for industrial-scale production are Zymomonas mobilis, Saccharomyces cervisiae, Escherichia coli, and Klebsiella oxytoca. Substantial attention and effort have been dedicated to redirecting the metabolic pathways of these and others towards higher yield of ethanol production, by means of metabolic engineering [16,17,18].

However, lack of suitable microorganisms that can efficiently convert the raw biomaterials to bio-ethanol has been one of the main obstacles to widespread use of bio-fuels. In addition to the ability to ferment a wide variety of sugars, some other features must be considered when choosing organisms for industrial-scale bio-ethanol production. These important features include but are not limited to the ability to have high ethanol yield, tolerance to fermentation products/by-products, simple growth requirements, and the ability to grow under conditions that prevent contaminating organisms from growing [14,16].

Production of bio-ethanol using thermophilic and hyperthermophilic organisms is the focus of many research groups. Extremophiles in general and hyperthermophiles in particular are outstanding organisms that produce highly stable enzymes due to their natural habitats, and many of them are able to tolerate changes in environment; making them good candidates for bio-ethanol production [19].

Several distinct advantages are associated with using thermophiles over mesophiles, including high temperatures and the mostly anaerobic nature of thermophilic organisms, which result in elimination of oxygenation and cooling of the fermenter. Another aspect is improved solubility of many reaction components at elevated temperatures [20]. In addition, the high temperature of the process leads to lowering the viscosity of reaction mixtures, causing improved production yields. Various thermophiles can ferment hexose and/or pentose sugars, as well as more complex substrates such as cellulose and xylan in some cases. Many of these organisms and their enzymes are relatively resistant to sudden pH or temperature changes and high concentrations of solvents [21,22,23]. High temperatures can result in lower gas solubility and significantly decrease the risk of process failure and product loss due to contamination that is the common problem in the yeast-based fermentation system. At the same time, high temperatures lower the cost of ethanol recovery due to the high volatility of ethanol at high fermentation temperatures [14,19,24,25]. However, there are some disadvantages associated with using hyperthermophiles, the most important one being their intrinsic low substrate tolerance and product/by-product inhibition [26,27]. Moreover, some of these organisms are mixed-fermenters that result in production of sometimes too many types of products during growth [14,16].

Application of metabolic engineering approaches has had a great impact on elimination of the problems associated with using thermophiles, and led to development of strains with bio-ethanol yields that are almost equal to those of the yeast-based systems. Members of the genus Clostridium, especially thermophilic members such as Clostridium thermocellum, have been studied intensively due to their competence in production substantial amounts of ethanol, butanol and hydrogen [24,28,29]. Members of the genus Thermoanaerobacter, including T. ethanolicus, T. tengcongensis, and T. pentosaceus are extremely thermophilic bacteria that are well studied for their high ethanol production potential especially from pentoses [30,31,32,33,34]. The genus Geobacillus has been studied widely for bio-ethanol production potential [19,29,35,36]. Production of ethanol, although at lower concentrations, has also been reported for the extremely thermophilic Caldicellulosiruptor species that includes C. owensensis [37], C. kristjanssonii [38], and C. saccharolyticus [39].

Compared to the thermophilic ethanol producers, very little is known about the ethanol production levels and pathways in the extremely thermophilic and hyperthermophilic microorganisms. It was shown that the peptide- and carbohydrate-fermenting hyperthermophilic archaeon Pyrococcus furiosus can produce H₂, CO₂, acetate, alanine, and small amounts of ethanol [40]. The strictly anaerobic archaeon Thermococcus sp. strain ES1 produced some ethanol and butanol when cultures were grown at low concentrations of elemental sulfur [41]. The production of ethanol as an end product of fermentation was also shown in the hyperthermophilic anaerobic archaeon Thermococcus guaymasensis [42] and more recently in the autotrophic hyperthermophile, Thermococcus onnurineus [43]. Within the bacterial hyperthermophiles, traces of ethanol have been reported in cultures of different Thermotogales including T. hypogea [44], T. lettingae [45], T. neapolitana [46], Kosmotoga olearia [47], and Thermosipho affectus [48].

3. Key Enzymes Involved in Ethanol Production

One of the key enzymes in both ethanol production pathways is alcohol dehydrogenase. Alcohol dehydrogenases are members of the oxidoreductase family and are present in all three domains of life [49,50]. They belong to the dehydrogenase/reductase superfamily of enzymes and catalyze the reversible inter-conversion of alcohols to corresponding aldehydes or ketons. ADHs can be classified based on their cofactor requirements: (I) the flavin adenine di-nucleotide (FAD)-dependent ADHs; (II) the pyrollo-quinoline quinone (PQQ), heme or cofactor F₄₂₀ dependent ADHs; (III) NAD(P)-dependent ADHs [49,51]. Alternatively, they can be divided into three major groups based on their molecular size and metal contents: the first group is known as zinc-dependent long chain alcohol dehydrogenase; which have sizes of 300–900 amino acids, the second group is the short chain alcohol dehydrogenase: which contain no metal ions and have approximate lengths of 250 amino acids; and the third group is the long-chain iron dependent ADHs; with a length of 385–900 residues [49,50,51,52].

Many different ADHs have been characterized from various thermophilic and hyperthermophilic bacteria and archaea, with a majority of them being NAD(P)-dependent. Some of the more recently characterized hyper/thermophilic ADHs are those from P. furiosus [53,54], Thermococcus hydrothermalis [55], Thermococcus kodakarensis [56,57,58], Thermococcus sibiricus [59,60], Thermococcus guaymasensis [42], Sulfolobus acidocaldarius [61], Thermococcus strain ES1 [62], Aeropyrum pernix [63], Thermotoga hypogea [64], and Pyrobaculum aerophilum [65].

Although there is a relatively long list of ADHs isolated and characterized from thermophilic and hyperthermophilic archaea and bacteria, with the physiological roles of several proposed to be in the reduction of aldehydes to alcohols, other enzymes involved in the ethanol production pathways are not well characterized, especially the enzyme(s) that catalyze the production of acetaldehyde from pyruvate.

4. Pathways for the Production of Acetaldehyde from Pyruvate

Pyruvate is an intermediate in the central metabolism of carbohydrates [66,67], and it can be converted to acetaldehyde that will eventually be reduced to ethanol using one of the following two pathways:

(1) A two-step pathway that is used by yeast and a few bacteria like Zymomonas mobilis [68] and Sarcina ventriculi [69]. In this pathway pyruvate is non-oxidatively decarboxylated to acetaldehyde and carbon dioxide, which is catalyzed by pyruvate decarboxylase (PDC). Acetaldehyde is then converted to ethanol that is catalyzed by ADH (Figure 1);

Figure 1.

Two pathways of ethanol production from pyruvate. POR; Pyruvate ferredoxin oxidoreductase; PFL; Pyruvate formate lyase, AcDH; Acetaldehyde dehydrogenase, ADH; Alcohol dehydrogenase, PDC; pyruvate decarboxylase; CoASH; coenzyme A, Fd_ox; oxidized ferredoxin, Fd_red; reduced ferredoxin.

(2) A three-step pathway that is more widespread in bacteria. Pyruvate is oxidatively decarboxylated to acetyl-coenzyme A (acetyl-CoA) by the metalloenzyme pyruvate ferredoxin oxidoreductase (POR) and/or pyruvate formate lyase (PFL). Acetyl-CoA is then converted to acetaldehyde by a CoA-dependent-acetylating acetaldehyde dehydrogenase (AcDH). Finally, acetaldehyde is reduced to ethanol by ADH.

The key metabolite for the two known pathways is acetaldehyde. The thiamine pyrophosphate (TPP)-dependent enzyme pyruvate decarboxylase is the only enzyme proficient at direct conversion of pyruvate to acetaldehyde. Interestingly, a majority (but not all) of the enzymes which are involved in the acetaldehyde production pathways are members of the superfamily of TPP-dependent enzymes, which includes PDC, POR, and PFL [70,71].

TPP, also known as thiamine diphosphate (ThDP), is composed of an aromatic methylaminopyrimidine ring, linked to a methyl thiazolium ring via. a methylene group with a pyrophosphate group attached to a hydroxylethyl side chain. TPP is derived from the water-soluble vitamin B1 and is the most common cofactor for enzymes that catalyze the cleavage and formation of carbon-carbon bonds next to a carbonyl group; hence TPP-dependent enzymes are involved in a wide range of metabolic pathways. Unlike many other cofactors (e.g., nicotinamide adenine dinucleotide, NADH) which are basically co-reactants, TPP remains at the enzymes’ catalytic center and is directly involved in the catalysis of the reaction [72]. The reactions catalyzed by TPP-dependent enzymes can be divided into at least three groups: the oxidative reactions, non-oxidative reactions, and carboligation reactions [73].

5. Pyruvate Decarboxylase (PDC)

In 1911, Neuberg and Karczag for the first time described the decarboxylation of pyruvate to acetaldehyde in S. cerevisiae. In 1922, the same research group detected the potential of yeast in the formation of C-C bonds. Neuberg named the new enzyme “carboligase”, and assumed it to exist apart from “α-carboxylase” (PDC) in yeast [74]. However, the preliminary characterization of the enzymes’ cofactor was delayed until 1937, when Lohmann and Schuster analyzed structure of the enzymes’ cofactor to be “cocarboxylase” or “aneurinpyrophosphate” or thiamin diphosphate [73].

The enzyme catalyzes non-oxidative decarboxylation of α-keto acids to produce a corresponding aldehyde and carbon dioxide. The most extensively examined enzymes of this group are the ones from Saccharomyces cerevisiae and its bacterial counterpart Z. mobilis. In addition to decarboxylation of pyruvate, PDC also catalyzes the enantio-selective formation of 2-hydroxy ketons via. carboligase side reactions.

PDC, or its gene (pdc), is found to be widely distributed in fungi and higher plants but it is relatively rare in prokaryotes and unknown in animals. In fungi, PDC is found in Saccharomyces cerevisiae, Saccharomyces carlsbergensis (also known as S. pastorianus) and Saccharomyces uvarum, Neurospora crassa, members of the Kluyveromyces species, members of the Aspergillus species, Hanseniaspora uvarum, Schizosaccharomyces pombe, and in Candida (Torulopsis) glabrata. PDC is present in a variety of plants, including maize (Zea maize), parsnip, orange, pea (Pisum sativum), jack bean, sweet potato, wheat, cotton wood, soybean and rice (Oryza sativa). In prokaryotes, PDC is found and studied in Z. mobilis, Sarcina ventriculi, Clostridium botulinum, Acetobacter species, Zymobacter palmae, and in Erwinia amylovora [75,76,77,78,79]. So far there has been no report on finding PDC/pdc homolog in thermophilic or hyperthermophilic bacteria or in any of the members of the third major evolutionary lineage of life, archaea as a whole [76,77,78,80].

PDCs from different organisms show at least a 30% identity at the amino acid level and most of them are composed of subunits of 562–610 amino acid residues. The holoenzyme is usually composed of four identical or non-identical subunits of approximately 60 kDa (ensuing in a total mass of about 240 kDa) in which every two subunits binds tightly (but not covalently) to a set of cofactors including TPP and Mg²⁺ ion. PDCs with four subunits are often arranged as a dimer of dimers, with multiple close contacts within the dimers and several contacts between the dimers. The contact area between two related dimers forms the “V” conformation that is a common property of all TPP-dependent enzymes studied so far, and it also has an essential role in cofactor binding for this group of enzymes [81,82].

The catalytic mechanism of PDC for the most part follows the principles of catalytic mechanisms of other TPP-dependent enzymes: in brief, carbonyl addition of pyruvate to the reactive C2 atom of the cofactor thiazolium ring [73] yields the intermediate 2-(2-lactyl)-TDP (LTDP). The subsequent release of carbon dioxide produces resonating carbanion/enamine forms of 2-(1-hydroxyethyl)-TDP (HETDP, also known as hydroxyethylidene-TPP). The resonating form is considered to be a central and highly reactive intermediate state in TPP-dependent enzymes acting on pyruvate. However, unlike most other TPP-dependent enzymes in which the intermediate is oxidized, the carbanion/enamine in PDC is protonated at the C2α position, yielding C2α-hydroxylethylthiamine diphosphate (HETDP) before the final release of acetaldehyde completes the reaction [72,83,84].

Crystal structures of several pyruvate decarboxylases are solved particularly from yeasts and Z. mobilis [81,85,86,87]. The active sites of these enzymes are also studied comprehensively using site-directed mutagenesis [88,89,90].

6. Pyruvate Ferredoxin Oxidoreductase (POR)

The enzyme pyruvate ferredoxin oxidoreductase (also known as pyruvate synthase as the reaction is reversible) is one of the best studied members of the 2-oxoacid oxidoreductase family [91,92,93]. The enzyme catalyzes coenzyme A and TPP-dependent oxidative decarboxylation of pyruvate to acetyl-CoA, releasing a molecule of CO₂ and transferring the reducing equivalents to the electron acceptor ferredoxin or flavodoxin. Alternatively, in other pyruvate oxidizing enzymes, the reducing equivalents are transferred to NAD⁺ (in the case of pyruvate dehydrogenase using lipoate as oxidizing agent for the production of acetyl-CoA), to molecular-oxygen-producing hydrogen peroxide (in the case of pyruvate oxidase), or to the carbonyl groups producing formate (in case of pyruvate formate lyase) [94,95,96]. In acetaldehyde- and ethanol-producing organisms, acetyl-CoA is usually converted to acetaldehyde via. the CoA-dependent (acetylating) acetaldehyde dehydrogenase.

POR uses iron-sulfur cluster chemistry to catalyze the pyruvate decarboxylation and release of acetyl-CoA. POR is an ancient molecule, and it seems to have existed even before the divergence of the domains of the bacteria and archaea [97]. The enzyme is present in all three domains of life. All archaea catalyze the conversion of pyruvate to acetyl-CoA using POR, and all of the archaeal genomes sequenced so far contain hetero-tetrameric PORs, which have been proposed to be the closest to the POR common ancestor [97,98].

POR is prevalent mainly in anaerobic bacteria and infrequently found in anaerobic protozoa, for example, in Giardia duodenalis [99] and Enthamoeba histolytica [100,101]. The enzyme has been isolated and studied from many different anaerobic or microaerophilic microorganisms including anaerobic bacteria like the genera Clostridium [102], Moorella thermoacetica [103] and anaerobic sulphate-reducing bacteria Desulfovibrio africanus [104,105,106,107]. In hyperthermophiles, PORs are characterized from the hyperthermophilic bacterium Thermotoga maritima [108] and hyperthermophilic archaea Pyrococcus furiosus [109] and Archaeoglobus fulgidus [110], as well as the methanogenic archaea Methanosarcina barkeri [111,112] and Methanobacterium thermoautotrophicum [113].

The quaternary oligomeric structure of the POR is variable depending on the source microorganism and can be homo-dimeric (e.g., most bacterial PORs), hetero-dimeric (e.g., POR of Halobacterium salinarium), hetero-tetrameric (archaeal PORs), and heteropentameric (anabolic PORs), although all of the PORs studied so far, regardless of their source and structure, seem to be phylogenetically related and derived from a common archaeal-type heterotetrameric ancestor [97,98].

The crystal structures of several POR have been determined. PORs from Desulfovibrio africanus (with and without bound substrate) and Desulfovibrio vulgaris [114,115,116] are among the most extensively studied PORs. POR is a metalloenzyme and all PORs studied so far contained between one and three [4Fe-4S] clusters arranged in a spatial order from the TPP located at the active center of the enzyme toward its surface, suggesting that they are part of an electron transfer pathway [117,118].

POR can also catalyze the reaction to form pyruvate from acetyl-CoA and carbon dioxide, which is the basis of the carbon dioxide fixation in many autotrophic microorganisms [119]. This type represents the so-called “anabolic” PORs that are studied from the thermophilic facultative aerobic bacterium Hydrogenobacter thermophilus [120,121,122], as well as the hydrogenotrophic methanoarchaeon Methanococcus maripaludis [123,124]. Interestingly, in the case of the heteropentameric POR of M. maripaludis, four subunits are very closely related to the archaeal heterotetrameric (ancestral) PORs, the fifth subunit has no known homologue within PORs.

The general steps of the POR catalytic reactions follow the same principles as those of other TPP-dependent enzymes. However, the enzyme is unique in one aspect: unlike most other TPP-dependent enzymes, POR takes advantage of free radical chemistry to catalyze the decarboxylation reaction [94,125].

Pyruvate dehydrogenase complexes (PDH), which also catalyze the oxidative decarboxylation of pyruvate to acetyl-CoA using NAD⁺ as an electron acceptor, are normally present in aerobic organisms [126,127], which have been found in some thermopiles [128,129,130]. But no PDH has been identified in hyperthermophiles [131]. Since PDH does not play any significant role in the production of acetaldehyde and ethanol, no further description of this enzyme complex will be given in this review.

7. POR/PDC Bi-Functional Enzyme

In 1997, it was reported that the POR was also capable of converting pyruvate to acetaldehyde in the hyperthermophilic anaerobic archaeon Pyrococcus furiosus [80]. Unlike the commonly-known PDCs, which employ chemical rather than radical intermediates and therefore are oxygen insensitive, the reported PDC activity was highly oxygen sensitive. Both the POR and PDC activities of the hyperthermophilic enzyme were TPP- and coenzyme A-dependent. By using the coenzyme A analogue (desulfocoenzyme A), it was shown that coenzyme A has only a structural, and not a catalytic role in the catalyzed PDC reaction. Consequently, a “switch” mechanism was proposed for the enzyme’s bi-functionality, suggesting the conversion of active aldehyde to either acetyl-CoA or acetaldehyde, depending on the binding of CoA. According to the proposed model, binding of coenzyme A causes conformational changes in the intermediate structure, causing its protonation and generation of hydroxyethyl-TPP (HETDP). This reaction leads to release of acetaldehyde, allowing for the regeneration of TPP and possible release of CoA [80]. Ferredoxin is not required for its full PDC activity nor has any inhibitory effect when tested under in vitro conditions. However, it is likely that the in vivo PDC activity might be dependent on the availability of oxidized ferredoxin, which means that a lower ratio of oxidized to reduced ferredoxin may favor the PDC activity and vice versa. Therefore, it can be predicted that more acetaldehyde would be produced for being reduced to ethanol if the ratio of the oxidized to reduced ferredoxin would be kept very low under the anaerobic growth condition. Such a low ratio may also require a relatively low activity of the ferredoxin-oxidizing hydrogenases present in P. furiosus [132,133].

To date there has been no further study on the bi-functionality of the POR enzyme or the physiological relevance of such bifunctionality in any other organisms. It is not clear whether this bi-functionality is only a trait of Pyrococcales’ POR or a common property of all hyper/thermophilic PORs.

8. Acetaldehyde Dehydrogenase (CoA-Acetylating)

Acetaldehyde dehydrogenase (CoA-acetylating, EC 1.2.1.10) is a member of a very divergent superfamily of enzymes known as the “aldehyde dehydrogenases”. The prototype enzyme (adhE) was first discovered in Escherichia coli and is required for its anaerobic growth [134]. It was then discovered in the strictly anaerobic bacterium Clostridium kluyveri [135]. The enzyme is responsible for the conversion of acetyl-coenzyme A (acetyl-CoA) to acetaldehyde that is eventually converted to ethanol. Two forms of the enzyme are available: one is the monofunctional enzyme with only AcDH activity (mhpF) and the other is the bifunctional enzyme with both AcDH and ADH activities (adhE). The latter group is composed of an ADH active C-terminal and an AcDH active N-terminal, a structure believed to be the result of gene fusion between the genes encoding for each single enzyme [136,137].

Reports are available on isolation and characterization of the bifunctional NADP-dependent alcohol/acetaldehyde dehydrogenase (CoA-acetylating) from mesophilic microorganisms including Giardia lamblia [138] and Enthamoeba histolytica [101,139]. They are also present in some thermophiles, including T. ethanolicus [140,141], T. mathranii [32] and members of the genus Geobacillus [19]. However, no mono- or bi-functional AcDH activity was characterized from hyperthermophiles. Survey of the fully sequenced genomes of hyperthermophilic archaea and bacteria has shown no adhE or mhpF homologue either (Eram and Ma, unpublished data).

9. Conclusions

Many hyperthermophilic microorganisms produce ethanol as an end metabolic product. Although alcohol dehydrogenase and pyruvate ferredoxin oxidoreductase are found to be present, enzymes catalyzing the production of acetaldehyde from pyruvate are not well characterized. The commonly-known pyruvate decarboxylase and coenzyme A-dependent aldehyde dehydrogenase have not been identified. The only report of a bi-functional pyruvate decarboxylase is the POR/PDC from P. furiosus, which is thermostable but oxygen-sensitive. Therefore, it is likely that they use a two-step pathway to convert pyruvate to ethanol. The regulation of each of the POR/PDC activities is not clear but it may be related to the redox states inside the cells. To date, it appears that at least one multifunctional enzyme is responsible for catalyzing the non-oxidative decarboxylation of pyruvate to acetaldehyde in hyperthermophiles, and further study is needed to understand the catalysis of acetaldehyde production from pyruvate at high temperatures.

Conflicts of Interest

The authors declare no conflict of interest.