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. 1995 Dec 19;92(26):12156–12159. doi: 10.1073/pnas.92.26.12156

Isolation of HLA-DR1.(staphylococcal enterotoxin A)2 trimers in solution.

R E Tiedemann 1, R J Urban 1, J L Strominger 1, J D Fraser 1
PMCID: PMC40315  PMID: 8618863

Abstract

Mutational studies indicate that the superantigen staphylococcal enterotoxin A (SEA) has two separate binding sites for major histocompatibility complex (MHC) class II molecules. Direct evidence is provided here for the formation of SEA-MHC class II trimers in solution. Isoelectric focusing separated SEA-HLA-DR1 complexes into both dimers and HLA-DR1.SEA2 trimers. The molar ratio of components was determined by dual isotope labeling. The SEA mutant SEA-F47S, L48S, Y92A, which is deficient in MHC class II alpha-chain binding, formed only dimers with HLA-DR1, whereas a second SEA mutant, SEA-H225A, which lacks high-affinity MHC class II beta-chain binding was incapable of forming any complexes. Thus SEA binding to its MHC receptor is a two-step process involving initial beta-chain binding followed by cooperative binding of a second SEA molecule to the class II alpha chain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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