Figure S2.

Dx and Su(dx) Induce Notch Endocytosis by Different Routes, Related to Figure 2

(A) Using N antibody uptake assay, Dx induced internalisation of Notch is reduced compared to control by Dynamin, Clathrin Heavy Chain and Synaptojanin RNAi (upper panels in B). In contrast Su(dx) induced Notch endocytosis is prevented by Dynamin but not Clathrin Heavy Chain or Synaptojanin RNAi (lower panels).

(B) Scoring of Notch localisation as % cells with mainly plasma membrane localisation (PM), vesicular localisation (V) or a mixture of plasma membrane and vesicular (PM=V). Data displayed as mean ±SEM from minimum of 3 repeats, each scoring minimum of 60 cells, ^∗ indicates p<0.05 compared to GFP RNAi control (Student t-test).