Skip to main content
. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: Oncogene. 2013 Nov 25;33(48):5511–5522. doi: 10.1038/onc.2013.493

Figure 1. Overexpression of Separase induces mammary tumor in MMTV-Espl1 mice.

Figure 1

(A) Kaplan–Meier analysis for tumor onset and survival for wild type (WT), MMTV-Espl1 (Tg1 and Tg2), MMTV-Espl1 virgin, MMTV-Espl1, p53+/−, and p53+/− genotypes. The number of mice used (n) for each genotype is shown in the bracket. Log-rank test p=0.3503. (B) Expression of Separase protein in MMTV-Espl1 virgin [lanes 1, 2: protein from whole cell lysate; lanes 3, 4: protein from primary mammary epithelial cells (MEC) at passage zero], primiparous at day 5 lactating (D5 1p), and multi-parous at day 5 lactating (D5 2p and D5 mp) mice. (C) Expression of Separase and its cohesin substrate Rad21 in virgin, multiparous WT and MMTV-Espl1 mammary gland of 7.5 months old mice, and in the mammary tumors from MMTV-Espl1 and MMTV-Espl1, p53+/− multiparous animals (n=3-5/group). p84 nuclear matrix protein is shown to compare loading. *anticipated Rad21 cleavage band.