NEUROSCIENCE Correction for “Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell–derived neocortex,” by Taisuke Kadoshima, Hideya Sakaguchi, Tokushige Nakano, Mika Soen, Satoshi Ando, Mototsugu Eiraku, and Yoshiki Sasai, which appeared in issue 50, December 10, 2013, of Proc Natl Acad Sci USA (110:20284–20289; first published November 25, 2013; 10.1073/pnas.1315710110).
The authors note that Fig. 2 appeared incorrectly. The corrected figure and its legend appear below.
Fig. 2.
Asymmetric rounding morphogenesis in self-organized cortical NE. (A–I) Asymmetric progression of rounding morphogenesis of hESC-derived cortical NE. Arrows, boundary of a cortical NE domain in A and rolling epithelium in (B–D). Red arrowheads, rolling epithelium in E. Red arrows, rounding movements of the NE (F–I). (J–L) Effect of the ROCK inhibitor Y-27632 on the rolling of cortical NE. (L) Attenuation of rolling morphogenesis with ROCK inhibitor. ***P < 0.001 in contingency table analysis (2 × 2) with Fisher’s exact test. Treatment group, n = 187 NE domains; control group, n = 130. (M and N) The rolling shape was preferentially observed on the side with strong expression of Otx2 and Coup-TF1 (dorsal and caudal markers). (O–Q) Adjacent formation of NE structures of cortex (Pax6+) and LGE (Gsh2+; with GAD65+ GABAergic neurons underneath) on day 35. The cortical side contacting the LGE domain was opposite to the side with strong rolling (arrow). (R) Interkinetic nuclear migration in the hESC-derived cortical NE on day 24 (two-photon imaging). Visualized with partial mixing of pax6::venus reporter hESCs with nonlabeled hESCs. Two daughter cells with both apical and basal processes were generated from an apically dividing progenitor (red dots). (Scale bars, 200 μm in A; 100 μm in B–H, J, K, M, and N; 200 μm in O–Q.) Nuclear counter staining (blue), DAPI.