Extended Data Figure 9.
Expression of SpmXAB(L)-EGFP or SpmXAE(S)-EGFP in C. crescentus does not induce ectopic stalk synthesis. (a-b) The localization of SpmXCC(P)-EGFP/SpmXCC(P)-mCherry and the phenotypes of the strains are identical. (c-e) Asticcacaulis SpmX-EGFP variants localize mostly to the pole in wild-type C. crescentus (left) and C. crescentus spmX− (right) strains. No ectopic stalks were observed, indicating that the alternative targets are lacking in C. crescentus. It should also be noted that the expression of variants of SpmXAE-EGFP can cause cell filamentation. (f) SpmXCC-EGFP localizes to the pole in the A. biprosthecum spmX− strain and cannot rescue stalk synthesis. All data are representative of three biological repetitions analyzing at least 100 cells each. Scale bars, 1μm.