It is important to note that Bédard and colleagues have also described a method for the single trial extraction of synaptic input (Bédard et al., 2012). This method, which requires that the membrane voltage remains subthreshold, thus in a linear domain, obtains an estimate for synaptic inputs based on time derivatives of passive recordings of the membrane voltage trace, i.e., without perturbations from injected current by the amplifier.
Conflict of interest statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
- Bédard C., Béhuret S., Deleuze C., Bal T., Destexhe A. (2012). Oversampling method to extract excitatory and inhibitory conductances from single-trial membrane potential recordings. J. Neurosci. Methods 210, 3–14 10.1016/j.jneumeth.2011.09.010 [DOI] [PubMed] [Google Scholar]