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. 1995 Dec 19;92(26):12367–12369. doi: 10.1073/pnas.92.26.12367

Genetic heterogeneity in isogenic homozygous clonal zebrafish.

D G Buth 1, M S Gordon 1, I Plaut 1, S L Drill 1, L G Adams 1
PMCID: PMC40358  PMID: 8618902

Abstract

The C32 isogenic homozygous diploid (IHD) strain of the zebrafish (Danio rerio) was found to be polyallelic at a malate dehydrogenase locus (sMdh-A). A variant allele is thought to have arisen via mutation within the past 10 bisexual generations that have maintained the strain since its last gynogenetic cloning event; this unique allele now predominates at the sMdh-A locus. The estimated mutation rate in this species is sufficiently high that long-term genetic homogeneity of its IHD clones cannot be assumed. Researchers using such bisexually maintained clones should be aware that they are not necessarily using genetically uniform subjects. Genetic uniformity of cloned IHD zebrafish will be maximized if experimental subjects are obtained soon after a cloning event.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barinaga M. Zebrafish: swimming into the development mainstream. Science. 1990 Oct 5;250(4977):34–35. doi: 10.1126/science.2218513. [DOI] [PubMed] [Google Scholar]
  2. Goff D. J., Galvin K., Katz H., Westerfield M., Lander E. S., Tabin C. J. Identification of polymorphic simple sequence repeats in the genome of the zebrafish. Genomics. 1992 Sep;14(1):200–202. doi: 10.1016/s0888-7543(05)80309-x. [DOI] [PubMed] [Google Scholar]
  3. Johnson S. L., Midson C. N., Ballinger E. W., Postlethwait J. H. Identification of RAPD primers that reveal extensive polymorphisms between laboratory strains of zebrafish. Genomics. 1994 Jan 1;19(1):152–156. doi: 10.1006/geno.1994.1026. [DOI] [PubMed] [Google Scholar]
  4. Kahn P. Zebrafish hit the big time. Science. 1994 May 13;264(5161):904–905. doi: 10.1126/science.8178149. [DOI] [PubMed] [Google Scholar]
  5. Meyer A., Biermann C. H., Ortí G. The phylogenetic position of the zebrafish (Danio rerio), a model system in developmental biology: an invitation to the comparative method. Proc Biol Sci. 1993 Jun 22;252(1335):231–236. doi: 10.1098/rspb.1993.0070. [DOI] [PubMed] [Google Scholar]
  6. Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics. 1978 Jul;89(3):583–590. doi: 10.1093/genetics/89.3.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Plaut I, I, Gordon M. SWIMMING METABOLISM OF WILD-TYPE AND CLONED ZEBRAFISH BRACHYDANIO RERIO. J Exp Biol. 1994 Sep;194(1):209–223. doi: 10.1242/jeb.194.1.209. [DOI] [PubMed] [Google Scholar]
  8. Postlethwait J. H., Johnson S. L., Midson C. N., Talbot W. S., Gates M., Ballinger E. W., Africa D., Andrews R., Carl T., Eisen J. S. A genetic linkage map for the zebrafish. Science. 1994 Apr 29;264(5159):699–703. doi: 10.1126/science.8171321. [DOI] [PubMed] [Google Scholar]
  9. Streisinger G., Walker C., Dower N., Knauber D., Singer F. Production of clones of homozygous diploid zebra fish (Brachydanio rerio). Nature. 1981 May 28;291(5813):293–296. doi: 10.1038/291293a0. [DOI] [PubMed] [Google Scholar]
  10. Turner B. J., Elder J. F., Jr, Laughlin T. F., Davis W. P. Genetic variation in clonal vertebrates detected by simple-sequence DNA fingerprinting. Proc Natl Acad Sci U S A. 1990 Aug;87(15):5653–5657. doi: 10.1073/pnas.87.15.5653. [DOI] [PMC free article] [PubMed] [Google Scholar]

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