There is an error in Table 1. The primer sequence for Heat-shock protein 70 (hsp 70) should be:
Table 1. Amplicon size, analyzed sequence fragment length and primer sequence of the target regions for the six loci studied.
| Locus | Amplicon size (bp) | Analyzed sequence length (bp) | Primer sequence (5’-3’) |
| 6-phosphogluconate dehydrogenase (6pgd) | 836 | 666 | CTCAAGGAACATGAGCACGA TTGTCCTTGACTTGCTCACG |
| Manose-6-phosphate isomerase (mpi) | 681 | 569 | GGCAAGATGTATGCGGAGTT CTCCCCAGGAACCATCTGTA |
| Isocitrate dehydrogenase (icd) | 1022 | 755 | GAATCGGGAAGGAGATCACA CATCATAGCCCCAGAGAGGA |
| Heat-shock protein 70 (hsp70) | 1022 | 896 | GACGGTGCCTGCCTACTTCAA CCGCCCATGCTCTGGTACATC |
| Malate dehydrogenase mitochondrial (mdhmt) | 821 | 666 | TGCCGACCTCTTCCATATTC GAGTGAGGTGCGTCTTCACA |
| Malate dehydrogenase nuclear (mdhnc) | 1010 | 803 | TCACAACCGCAACTACGA CTACTCACGATAACGGCAGA |
Forward: GAC GGT GCC TGC CTA CTT CAA, Reverse: CCG CCC ATG CTC TGG TAC ATC.
Reference
- 1. Marlow MA, Boité MC, Ferreira GEM, Steindel M, Cupolillo E (2014) Multilocus Sequence Analysis for Leishmania braziliensis Outbreak Investigation. PLoS Negl Trop Dis 8(2): e2695 doi:10.1371/journal.pntd.0002695 [DOI] [PMC free article] [PubMed] [Google Scholar]