Table 2. Superoxide Production by Isolated Spinach Cytochrome b6f Complex; Electron Acceptor, Plastocyanin.
Electron transfer rate, Cyt b6f-1 · s-1 | Superoxide production rate Cyt b6f-1 · s-1 | Superoxide production (% electron transfer rate) | |
---|---|---|---|
Control, no inhibitor | 245 ± 25 (n=10) | 4.5 ± 0.6 (n=10) | 1.9 ± 0.3 |
NQNO, 5 μM | 196 ± 7 (n=3) | 3.5 ± 0.4 (n=3) | 1.8 ± 0.2 |
DBMIB, 4 μM | n.d. (n=4) | n.d. (n=3) | - |
n, number of trials; n.d., rate too small to allow accurate determination.