Fig. 5.

Antibody responses in infected humans. (A) Sera from infected humans before (BT) and after treatment (AT) with praziquantel were used to probe the printed defined schistosome glycan microarray at a dilution of 1:1000. Data show one representative (ID 19) of five human serum samples. (B) Binding of pooled newly infected human sera and normal (unexposed) on the defined schistosome microarray bearing an additional core fucose epitope (ID 21, 22). ID numbers on x-axes correspond to glycopeptides listed in Table I. (C–F) Human sera samples (ID 11, 19, 26, 42, 59) before (B) and after treatment (A) were used to probe a CFG glycan microarray. IgM (C) and IgG (D) responses to LDN and LDNF, and IgM (E) and IgG (F) responses to various Le^X antigens are shown. Numbers in the legend for E and F correspond to the Le^X-containing glycans listed in Table II. Five human samples (IDs on x-axes) were analyzed plus a normal human serum (Unexp.) control.