Table 1.

Number and types of libraries and reads obtained in the Amazon Continuum Project, June 2010, R/V Knorr

	Metagenomes	Non-selective metatranscriptomes	Poly(A)-selective metatranscriptomes
Data type	Total community DNA	Total community mRNA	Eukaryotic community mRNAa
# Stations sampled	6	6	6
# Size fractions sampled	2	2	1
# Replicates	2	2	2
# Samples	24	24	12
# Raw reads	3.68 × 108	8.12 × 108	4.61 × 108
# Joined reads post QC	9.50 × 107	1.62 × 108	1.01 × 108
Average joined read length (bp)	205	190	185
# rRNA reads	-	9.53 × 107	2.34 × 105
# Potential protein-encoding reads	9.44 × 107	6.52 × 107	9.86 × 107

^aThe selective metatranscriptomes captured poly(A)-tailed transcripts and are therefore systematically biased against transcripts from eukaryotic organelles. #, number of; QC, quality control.