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. Author manuscript; available in PMC: 2015 May 23.
Published in final edited form as: Vaccine. 2014 Apr 8;32(25):3050–3057. doi: 10.1016/j.vaccine.2014.03.049

Figure 3. Immunization with rPIV5-RSV-F and rPIV5-RSV-G induce IgG1 and IgG2a antibody responses similar to RSV A2 infection.

Figure 3

Naïve BALB/c mice were treated with PBS or immunized intranasally with 106 PFU of rPIV5-RSV-F, rPIV5-RSV-G, or RSV A2. Sera were collected 3 weeks post-immunization. (A, B) RSV-F (A) and RSV-G-specific (B) IgG1 and IgG2a antibody curves were generated by ELISA using isotype-specific secondary antibodies conjugated to HRP. (C, D) RSV-F (C) and RSV-G-specific (D) IgG2a/IgG1 ratios were calculated using values in (A) and (B), respectively. IgG1 and IgG2a antibodies in the sera were quantified using standard curves to generate IgG2a/IgG1 ratios. Each group consisted of 5 mice. Solid horizontal lines represent the mean of each group. Dotted horizontal lines indicate a ratio of 1.