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. Author manuscript; available in PMC: 2014 Jun 2.
Published in final edited form as: Differentiation. 2009 Apr 15;77(5):442–449. doi: 10.1016/j.diff.2009.03.001

Figure 3. Cellular proliferation is intact in the small intestine of Mdm4intΔ mice.

Figure 3

(A) Ki-67 immunofluorescence in 3-day old small intestines. Orange fluorescence, cytokeratin (epithelial marker); turquoise fluorescence, Ki-67 (proliferation marker); blue fluorescence, TOPRO-3 (nuclear counterstain); original magnification X400. Graph represents the quantification of the Ki-67 positive staining. (B) Analysis of BrdU incorporation in 8-week old mouse intestines. Mice were injected with 100 µg BrdU/g body weight and sacrificed 2 hr later. BrdU labeled cells (brown) were quantified and values were graphed. The p-value was obtained using Student’s t-test (n = 3 per group); original magnification X200. (C) Reverse transcription RT-PCR analysis of the p53 target, p21. Five mice per group were analyzed and the fold change in p21 mRNA levels was graphed. The p-value was obtained using Student’s t-test.