CELL BIOLOGY. For the article “Cholesterol depletion induces PKA-mediated basolateral-to-apical transcytosis of the scavenger receptor class B type I in MDCK cells,” by Patricia V. Burgos, Carla Klattenhoff, Erwin de la Fuente, Attilio Rigotti, and Alfonso González, which appeared in issue 11, March 16, 2004, of Proc. Natl. Acad. Sci. USA (101, 3845-3850; first published March 8, 2004; 10.1073/pnas.0400295101), the authors note that the ordinate for the graphs in Fig. 5 B and C should read “cAMP levels (pmol/μg)” instead of “PKA activity (pmol/min/μg).” The corrected figure and its legend appear below.
Fig. 5.
Acute cholesterol depletion by MβCD activates PKA without increasing intracellular cAMP levels. MDCK cells were incubated at 37°C with 10 mM MβCD or 50 μM FSK. (A) Both treatments increased PKA activity. (B) In contrast with FSK, cells treated with MβCD showed undetectable levels of cAMP levels. (C) In the presence of increasing concentrations of phosphodiesterase inhibitor IBMX, cAMP levels elicited by 1 h of FSK treatment increased progressively whereas, during treatment with MβCD, they remained almost undetectable. Each point represents average and SE.