Volume 300, February 2011
Volume 69, February 2011
Johannessen M, Fontanilla D, Mavlyutov T, Ruoho AE, Jackson MB. Antagonist action of progesterone at σ-receptors in the modulation of voltage-gated sodium channels. Am J Physiol Cell Physiol 300: C328–C337, 2011. First published November 17, 2010; doi:10.1152/ajpcell.00383.2010 (http://ajpcell.physiology.org/content/300/2/C328).—In results, Fig. 1B is incorrect. The correct Fig. 1 is shown below. The article has been corrected online.
Fig. 1.
Progesterone binding to σ-receptors. A: radioligand binding curves demonstrating the competitive displacement by progesterone of ligand binding to σ1- and σ2-receptors. (+)[3H]-pentazocine was used as a ligand for σ1-receptors. [3H]ditolylguanidine ([3H]DTG) with cold (+)pentazocine (μM) was used to resolve σ2-receptors. The binding affinity of progesterone was determined to be 239 nM for σ1-receptors and 441 nM for σ2-receptors. B: σ-Receptor photolabeling with the σ1/σ2-receptor photolabel 1-N-(2′,6′-dimethyl-morpholino)-3-(4-azido-3-[125I]iodo-phenyl)propane ([125I]IAF) indicated that σ1- and σ2-receptors are expressed in human embryonic kidney (HEK)293 cells. The first lane represents HEK293 cell homogenates alone [control (Cont)], the second in 10 μM (+)pentazocine (Pent), the third in 20 μM DTG, and the fourth in 50 μM progesterone (Prog).
