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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Virology. 2014 Apr 15;0:205–219. doi: 10.1016/j.virol.2014.03.010

Figure 5.

Figure 5

Nuclear fragmentation is AIF-dependent. (A) Protein lysates from identical numbers of HeLa cells (Parental) or HeLa cells stably expressing an shRNA targeting bacterial beta galactosidase (shLac) or AIF (shAIF) were analyzed by immunoblotting for AIF and β-actin. The approximate migration of molecular weight standards (kDa) is indicated on the right. In panels B and C, the viruses indicated were used at an MOI of 10 to infect AIF-proficient (shLacZ) and AIF-deficient (shAIF) (B) HeLa and (C) PC3 cells. Cells were stained for DNA at 72 hpi and the fraction of cells containing fragmented nuclei determined. Results from a representative experiment of at least three experiments are shown. Error bars indicate the 95% exact binomial confidence intervals for the representative experiment.