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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Virology. 2014 Apr 10;0:171–178. doi: 10.1016/j.virol.2014.03.022

Fig. 2. Expression of the HK97 major capsid protein without the delta domain.

Fig. 2

A. Radiolabelled proteins produced by the wild-type expression plasmid and plasmid pNoDelta2 were analyzed on a SDS polyacrylamide gel. The control plasmid synthesizes both the protease and the mcp, gp5. B. SDS gel analysis of high-level expression of pNoDelta2. Protease− plasmid (pVB) has a protease knockout mutation and produces Prohead I composed of 42 kDa gp5. pNoDelta2 produces mcp at the expected size in the pellet fraction only (labeled with circle). C. Fractions from the high-level expression were run on a 0.8% agarose gel, and stained. Prohead, head and capsomer bands are indicated. The pNoDelta2 mcp did not produce any distinct capsid assembly structures. The background smears of protein in the supernatant fractions are from cell proteins, which are also present when an empty plasmid vector is expressed.