Skip to main content
. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Virology. 2014 Apr 3;0:96–107. doi: 10.1016/j.virol.2014.03.017

Figure 4. Efficiency of TLR3 signaling in cells expressing NS1 amino acid substitution proteins.

Figure 4

HeLa-NFκB-luc reporter cells stably transduced with lentivirus expressing either wt NS1, MCS, or single or double NS1 amino acid changes were treated with 20μg/ml pIC for 8h or left untreated. (A) IL-6 secretion in the supernatants of cells was determined by enzyme-linked immunosorbent assay (ELISA) and fold induction was calculated by comparing IL-6 produced by pIC treated cells to that of untreated cells. Data are the average of two independent experiments performed in biological triplicate and error bars represent standard deviation from the mean. Statistical significance was determined by Mann-Whitney U test, where *p≤0.05 NS= not significant. All samples were compared to wt NS1. (B) Fold NFκB induction was determined by luciferase reporter assay. Relative light units were measured and reported as fold induction over untreated cells. Data are the average of two independent experiments and error bars represent standard deviation from the mean. Statistical significance was determined by student’s t-test, where *p≤0.05 NS= not significant. All samples were compared to wt NS1.