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. 2014 Aug;143(100):41–51. doi: 10.1016/j.ultramic.2013.10.011

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© 2013 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).

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Fig. 4 — CryoLM and cryoEM correlation from low magnification to high magnification images of plunge-frozen U2OS cells infected with Ad5-488 labelled virus particles. (A1) A cryoEM low magnification montage (LMM) identifies the cell of interest (green rectangle in dashed yellow square). (A2) Shows a cryoLM phase contrast image for a subset of (A1). The arrows in (A1 and A2) point to the same grid marks. (B1) CryoEM high magnification montage (HMM) for the area indicated by the green rectangle in (A1/A2). (B2) Merged phase contrast (displayed in green) and blue FluoSpheres channel. (B3) Merged blue (FluoSpheres and TetraSpeck) and green (Ad5-488 virus and TetraSpeck) channel after multi-channel drift correction. The cell's shape, carbon mesh and FluoSpheres shown in B2 help in finding the relative orientation of HMM (B1) and cryoFM (B3) images. (C1-3) Zoom into areas indicated by red dashed squares in (B1-3), respectively. Highly fluorescent Ad5-488 viruses (marked by open arrowheads) were located in thin cellular areas. White and red circles indicate FluoSpheres and TetraSpeck microspheres respectively. Only microspheres close to the plasma membrane of the cell are circled in corresponding images. Scale Bars: (A1–A2) 100 μm; (B and C) 5 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).