There are 2 errors in Table 1. The following PDB codes are missing: 4K23 for ATN-658 Fab and 4K24 for ATN-658-uPAR-ATF-SMB. Please see below for a corrected version.
Table 1. Statistics of X-ray data collection and structural model refinement.
| Crystals | ATN 658 Fab | ATN-658-uPAR-ATF-SMB |
| X-ray source | APS SER-CAT beamline 22-ID | BNL x29 |
| Temperature (K) | 100 | 100 |
| Resolution range | 31.45-1.60 (1.64-1.60)a | 26.90- 4.50 (4.66-4.50) a |
| Wavelength (Å) | 1.04 | 1.04 |
| Space group | P1211 | H32 |
| Cell parameters (Å) | 37.31, 132.30, 46.83 | 164.65, 164.659, 391.582 |
| Unique reflections | 49312 (4021) | 12451 (1221) |
| Rmerge (%)b_ | 0.094 (0.290) | 0.139 (0.841) |
| Completeness (%) | 85.7(70.0) | 99.7(100.0) |
| Average I/σ | 9.2 (4.65) | 16.14 (2.03) |
| Data redundancy | 3.0 (2.6) | 4.4 (4.5) |
| Model refinement: | ||
| R-factor/Rfree (%) | 19.8/24.6 | 20.7/29.3 |
| Overall B-factors (Å2) | 23.0 | 61.436 |
| Protein B-factors (Å2) | 21.3 | 259.694 |
| Solvent B-factors (Å2) | 39.4 | |
| R.m.s.d. from ideal bond length (Å) | 0.012 | 0.013 |
| R.m.s.d from ideal bond angles (°) | 1.407 | 2.018 |
| Ramachandran plot, % residues in regions: | ||
| favored | 93.8 | 84.5 |
| allowed | 5.7 | 13.7 |
| outlier | 0.5 | 1.8 |
| PDB ID | 4K23 | 4K24 |
a
Numbers in the parentheses are for the highest resolution shells; b Rmerge = ΣhΣi|Ii(h)-<I(h)>|/ΣhΣiIi(h), where <I(h)> is the mean intensity of reflection h.
References
- 1. Xu X, Cai Y, Wei Y, Donate F, Juarez J, et al. (2004) Identification of a New Epitope in uPAR as a Target for the Cancer Therapeutic Monoclonal Antibody ATN-658, a Structural Homolog of the uPAR Binding Integrin CD11b (αM). PLoS ONE 9(1): e85349 doi:10.1371/journal.pone.0085349 [DOI] [PMC free article] [PubMed] [Google Scholar]