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. 1995 Dec 5;92(25):11701–11705. doi: 10.1073/pnas.92.25.11701

Mapping by multifragment cloning in vivo.

D L Marykwas 1, S E Passmore 1
PMCID: PMC40470  PMID: 8524832

Abstract

An efficient method for mapping mutations is described in which hybrid genes, derived partly from mutant and partly from wild-type DNA, are obtained in vivo by homologous recombination of multiple fragments. The recombinants are formed in a strain in which their phenotypes are immediately apparent. This method was developed to identify changes that disrupt protein-protein interactions demonstrable by the two-hybrid system in yeast. However, it can be extended to any system where recombination is possible, provided an assay is available to distinguish between mutant and wild-type phenotypes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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