Abstract
The lactic acid bacterium Leuconostoc pseudomesenteroides can be found in mesophilic cheese starters, where it produces aromatic compounds from, e.g., citrate. Here, we present the draft genome sequences of two L. pseudomesenteroides strains isolated from traditional Danish cheese starters.
GENOME ANNOUNCEMENT
Here, we present the draft genome sequences of two Leuconostoc pseudomesenteroides strains, PS12 and 1159, which were isolated from two different Danish mesophilic undefined cheese starters (1). L. pseudomesenteroides is a versatile organism that has been isolated from various food sources (2, 3). It produces diacetyl and contributes to the eye formation in Gouda type cheese via its heterofermetative metabolism and ability to degrade citrate (4). Currently, there are two publicly available L. pseudomesenteroides draft sequences: those of strain 4882, isolated from a French dairy starter culture (5), and strain 3652T, isolated from cane juice (6). Sequencing libraries were prepared using the Nextera XT kit (Illumina, USA), according to the manufacturer’s recommendations, followed by sequencing as a part of the flowcell, as 2 × 250-base paired-end reads using the Illumina MiSeq (Illumina, USA) technology. The reads were trimmed and assembled with the CLC Genomics Workbench 6.5.1 (CLC bio, Denmark). The resulting contigs were annotated using the RAST server (7). The two strains have similar sizes and genomic features (Table 1) and share a number of conserved functions, including genes for central carbohydrate metabolism and protein degradation. Both strains also contain clustered regularly interspaced short palindromic repeat (CRISPR) elements, which were also found in L. pseudomesenteroides strain 4882 but not in strain 3652T. The finding that the main differences between the two genomes were coding sequences (CDS) for CRISPR elements and different phage genes indicates a prominent influence of phage exposure on the adaptation of these strains in dairy environments. Future work with the four genomes will give more insight on the evolution and adaptation of L. pseudomesenteroides to different environments.
TABLE 1.
General features for strains 1159 and PS12
| Feature |
L. pseudomesenteroides strain: |
|
|---|---|---|
| 1159 | PS12 | |
| Genome size (bp) | 2,038,943 | 1,935,842 |
| No. of open reading frames | 2,109 | 1,964 |
| G+C content (%) | 38.9 | 39.0 |
| No. of RNA genes | 48 | 47 |
| No. of contigs | 100 | 91 |
| Coverage (×) | 100 | 380 |
Nucleotide sequence accession numbers.
The whole-genome shotgun projects for L. pseudomesenteroides strains 1159 and PS12 have been deposited at DDBJ/EMBL/GenBank under the accession no. JAUI00000000 and JDVA00000000, respectively. The versions described in this paper are JAUJ01000000 and JDVA01000000, respectively.
ACKNOWLEDGMENTS
This work was supported by Copenhagen University.
We thank Taylor Oberg for technical assistance in genome annotation and assembly.
Footnotes
Citation Pedersen TB, Kot WP, Hansen LH, Sørensen SJ, Broadbent JR, Vogensen FK, Ardö Y. 2014. Genome sequences of two Leuconostoc pseudomesenteroides strains isolated from Danish dairy starter cultures. Genome Announc. 2(3):e00484-14. doi:10.1128/genomeA.00484-14.
REFERENCES
- 1. Pedersen TB, Ristagno D, McSweeney PLH, Vogensen FK, Ardo Y. 2013. Potential impact on cheese flavour of heterofermentative bacteria from starter cultures. Int. Dairy J. 33:112–119. 10.1016/j.idairyj.2013.03.003 [DOI] [Google Scholar]
- 2. Camu N, De Winter T, Verbrugghe K, Cleenwerck I, Vandamme P, Takrama JS, Vancanneyt M, De Vuyst L. 2007. Dynamics and biodiversity of populations of lactic acid bacteria and acetic acid bacteria involved in spontaneous heap fermentation of cocoa beans in Ghana. Appl. Environ. Microbiol. 73:1809–1824. 10.1128/AEM.02189-06 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3. Paulo EM, Boffo EF, Branco A, Valente AM, Melo IS, Ferreira AG, Roque MR, Assis SA. 2012. Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain. An. Acad. Bras. Cienc. 84:495–507. 10.1590/S0001-37652012000200018 [DOI] [PubMed] [Google Scholar]
- 4. Hemme D, Foucaud-Scheunemann C. 2004. Leuconostoc, characteristics, use in dairy technology and prospects in functional foods. Int. Dairy J. 14:467–494. 10.1016/j.idairyj.2003.10.005 [DOI] [Google Scholar]
- 5. Meslier V, Loux V, Renault P. 2012. Genome sequence of Leuconostoc pseudomesenteroides strain 4882, isolated from a dairy starter culture. J. Bacteriol. 194:6637–6637. 10.1128/JB.01696-12 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6. Kim DW, Choi SH, Kang A, Nam SH, Kim RN, Kim A, Kim DS, Park HS. 2011. Genome sequence of Leuconostoc pseudomesenteroides KCTC 3652. J. Bacteriol. 193:4299–4299. 10.1128/JB.05433-11 [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M, Meyer F, Olsen GJ, Olson R, Osterman AL, Overbeek RA, McNeil LK, Paarmann D, Paczian T, Parrello B, Pusch GD, Reich C, Stevens R, Vassieva O, Vonstein V, Wilke A, Zagnitko O. 2008. The RAST server: Rapid Annotations using Subsystems Technology. BMC Genomics 9:75. 10.1186/1471-2164-9-75 [DOI] [PMC free article] [PubMed] [Google Scholar]