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. Author manuscript; available in PMC: 2014 Jun 6.
Published in final edited form as: Neuron. 2013 May 22;78(4):623–630. doi: 10.1016/j.neuron.2013.03.021

Figure 2. Effect of GAPDH or C150S GAPDH on Behavioral and Neurotoxic Actions of Cocaine.

Figure 2

(A) Confocal microscopic analysis of the intrastriatal injection of lentiviral particles of HAGAPDH and HA-C150S GAPDH in mice brain. (B) Western blot analysis to detect overexpression of HA-GAPDH and HA-C150S GAPDH in brain. (C) Level of nitrosylation of GAPDH (SNO-GAPDH) in striatum of mice receiving single injections of cocaine (5–30 mg/kg). (D) Level of nitrosylation of GAPDH (SNO-GAPDH) in striatum of mice receiving cocaine (30–50 mg/kg for 5 days). (E and F) Levels of c-fos (E) and CREB binding to c-fos promoter (F) were measured in mice overexpressing HA-GAPDH or HA-C150S GAPDH in striatum. (G and H) PUMA protein level (G) and p53 binding to PUMA promoter (H) were measured in mice overexpressing GAPDH or GAPDH-C150S in striatum. *p < 0.01, n = 3, one-way ANOVA, mean ± SEM.