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. Author manuscript; available in PMC: 2014 Aug 20.
Published in final edited form as: Nature. 2014 Jan 5;506(7488):387–390. doi: 10.1038/nature12891

Figure 2.

Figure 2

IME2 is required for both mating and meiosis in C. lusitaniae. a, Deletion of IME2 blocks meiosis in C. lusitaniae (5-day time course, D1-D5). # represents p < 0.01, one way ANOVA, n=3. b , Wildtype diploid cells generate dyad spores (asterisks) after 3 days on PDA medium, whereas ime2Δ/ime2Δ diploids do not sporulate, n=3. c, RT-PCR data demonstrates induction of IME2 during C. lusitaniae mating, n=3. d, Loss of IME2 in both a and α cells leads to decreased mating (4 day time course, D1-D4). “#” represents P < 0.05, Kruskal Wallis, n=4. e, ime2Δ mutants generate mating zygotes, indicating the block to mating occurs post-cell fusion, n=4. Scale bars, 5 μm. Data represented as mean +/- s.e.m.